2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. NCGC00249987

NCGC00249987 is a highly selective and allosteric Tyr phosphatase activity of Eya2 inhibitor with IC50s of 3 μM and 6.9 μM for Eya2 ED and MBP-Eya2 FL. NCGC00249987 specifically targets migration, invadopodia formation, and invasion of lung cancer cells.

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NCGC00249987

NCGC00249987 Chemical Structure

CAS No. : 1384864-80-5

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Based on 1 publication(s) in Google Scholar

NCGC00249987 Related Antibodies

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Description

NCGC00249987 is a highly selective and allosteric Tyr phosphatase activity of Eya2 inhibitor with IC50s of 3 μM and 6.9 μM for Eya2 ED and MBP-Eya2 FL. NCGC00249987 specifically targets migration, invadopodia formation, and invasion of lung cancer cells[1].

IC50 & Target

IC50: 3 μM (Eya2 ED) and 6.9 μM (MBP-Eya2 FL)[1]
In Vitro

NCGC00249987 inhibits Eya2 ED (C-terminal Eya Domain). NCGC00249987 inhibits full length Eya2 including but not other Eya family members[1].
NCGC00249987 binding leads to a conformational change of the active site that is unfavorable for Mg2+ binding, thereby inhibiting Eya2’s Tyr phosphatase activity[1].
NCGC00249987 (5, 10 μM) treatment effectively suppresses migration, invadopodia formation and invasion in Eya2 WT expressing Calu-6 cells, but not in EV Calu-6 cells. NCGC00249987 does not inhibit cell growth or survival[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NCGC00249987 Related Antibodies
Molecular Weight

342.35

Formula

C16H11FN4O2S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

FC1=CC=CC(C(N/N=C/C(O2)=CC=C2SC3=NC=CC=N3)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (292.10 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9210 mL 14.6049 mL 29.2099 mL
5 mM 0.5842 mL 2.9210 mL 5.8420 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9210 mL 14.6049 mL 29.2099 mL 73.0247 mL
5 mM 0.5842 mL 2.9210 mL 5.8420 mL 14.6049 mL
10 mM 0.2921 mL 1.4605 mL 2.9210 mL 7.3025 mL
15 mM 0.1947 mL 0.9737 mL 1.9473 mL 4.8683 mL
20 mM 0.1460 mL 0.7302 mL 1.4605 mL 3.6512 mL
25 mM 0.1168 mL 0.5842 mL 1.1684 mL 2.9210 mL
30 mM 0.0974 mL 0.4868 mL 0.9737 mL 2.4342 mL
40 mM 0.0730 mL 0.3651 mL 0.7302 mL 1.8256 mL
50 mM 0.0584 mL 0.2921 mL 0.5842 mL 1.4605 mL
60 mM 0.0487 mL 0.2434 mL 0.4868 mL 1.2171 mL
80 mM 0.0365 mL 0.1826 mL 0.3651 mL 0.9128 mL
100 mM 0.0292 mL 0.1460 mL 0.2921 mL 0.7302 mL
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NCGC00249987 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NCGC002499871384864-80-5NCGC 00249987NCGC-00249987PhosphataseTyrphosphataseEya2EDMBP-Eya2FLmigrationinvadopodiaformationinvasionlungcancerC-terminalEyaDomain,Inhibitorinhibitorinhibit

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