NCP2 Anchor 

NCP2 Anchor can be used in the synthesis of exon jumping oligomer conjugates. The oligomer conjugates complement selected target sites in the human anti-muscular atrophy protein gene and induce exon 52 jumping. NCP2 Anchor can be used for research of muscular dystrophy^[1].

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
NCP2 Anchor Loaded Resin Synthesis^[1].
To a 75 L solid phase synthesis reactor with a Teflon stop cock was charged about 52 L of NMP and 2300 g of aminomethyl polystyrene resin.
The resin was stirred in the NMP to swell for about 2 hours then drained.
The resin was washed twice with about 4 L DCM per wash, then twice with 39 L Neutralization Solution per wash, then twice with 39 L of DCM per wash.
The NCP2 Anchor Solution was slowly added to the stirring resin solution, stirred for 24 hours at room temperature, and drained.
The resin was washed four times with 39 L of NMP per wash, and six times with 39 L of DCM per wash.
The resin is first treated with half of the DEDC capping solution and stirred for 30 minutes, then drained; then treated with the remaining half of the DEDC capping solution and stirred for 30 minutes, then drained.
The resin was washed four times with 39 L of NMP per wash, and six times with 39 L of DCM per wash.
The resin was treated and stirred with 1/2 the DEDC Capping Solution for 30 minutes, drained, and was treated and stirred with the 2nd ½ of the DEDC Capping Solution for 30 minutes and drained.
The resin was washed six times with 39 L of DCM per wash then dried in an oven to constant weight of 3573.71 g of Anchor Loaded Resin.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

676.71

C₃₈H₃₆N₄O₈

1380600-13-4

Solid

White to off-white

O=C(OC(CC1=CC=C(C(N2CCN(C(C3=CC=CC=C3)(C4=CC=CC=C4)C5=CC=CC=C5)CC2)=O)C=C1[N+]([O-])=O)C)ON6C(CCC6=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Gunnar J. Hanson, et al. Exon skipping oligomer conjugates for muscular dystrophy. US20190365919A1.