2. Apoptosis
  3. Necroptosis RIP kinase
  4. Necrostatin-5

Necrostatin-5 (Nec-5) is a potent necroptosis inhibitor with an EC50 value of 0.24 µM. Necrostatin-5 also is a RIP1 inhibitor. Necrostatin-5 shows cardioprotective effects.

For research use only. We do not sell to patients.

Necrostatin-5

Necrostatin-5 Chemical Structure

CAS No. : 337349-54-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Necrostatin-5 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Necrostatin-5

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Description

Necrostatin-5 (Nec-5) is a potent necroptosis inhibitor with an EC50 value of 0.24 µM. Necrostatin-5 also is a RIP1 inhibitor. Necrostatin-5 shows cardioprotective effects[1][2][3].

IC50 & Target[1][3]

RIPK1

 

necroptosis

0.24 μM (EC50)

Cellular Effect
Cell Line Type Value Description References
Jurkat EC50
0.24 μM
Compound: Nec-15; N13
Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 38199165]
Jurkat EC50
0.24 μM
Compound: Nec-5
Inhibition of TNF-alpha-induced necroptosis in FADD-deficient Jurkat T cells
Inhibition of TNF-alpha-induced necroptosis in FADD-deficient Jurkat T cells
[PMID: 17270434]
Jurkat EC50
240 nM
Compound: 3
Inhibition of cellular necroptosis in TNFalpha treated FADD deficient human Jurkat cells
Inhibition of cellular necroptosis in TNFalpha treated FADD deficient human Jurkat cells
[PMID: 18408713]
In Vitro

Necrostatin-5 (1, 5, 10, 50, 100 µM) inhibits LDH release in a dose-dependent mnner[3].
Necrostatin-5 (100 µM) protects MH-S cells against challenge with S. aureus, Listeria monocytogenes, S. pneumoniae, uropathogenic Escherichia coli (UPEC), and protectes against pneumolysin-induced death[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Necrostatin-5 Related Antibodies
In Vivo

Necrostatin-5 (2.46 mg/kg; i.p.) shows cardioprotective effects on the isolated heart model in rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 200-300 g, male Wistar rats[2]
Dosage: 2.46 mg/kg
Administration: I.p.; 60 min before experiment
Result: Reduced the infarction zone caused by 30-min global ischemia and 120-min reperfusion.
Molecular Weight

383.49

Formula

C19H17N3O2S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N1C2=CC=C(C=C2)OC)C3=C(N=C1SCC#N)SC4=C3CCCC4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (13.04 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6076 mL 13.0381 mL 26.0763 mL
5 mM 0.5215 mL 2.6076 mL 5.2153 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6076 mL 13.0381 mL 26.0763 mL 65.1907 mL
5 mM 0.5215 mL 2.6076 mL 5.2153 mL 13.0381 mL
10 mM 0.2608 mL 1.3038 mL 2.6076 mL 6.5191 mL
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Necrostatin-5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Necrostatin-5337349-54-9Nec-5Necrostatin5Necrostatin 5Nec5Nec 5NecroptosisRIP kinaseReceptor-interacting protein kinasesRIPKLDHcardioprotectiveMH-S cellsInhibitorinhibitorinhibit

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