NFYi5 

NFYi5 is a nuclear transcription factor-Y (NF-Y) inhibitor. NFYi5 disrupts the binding of NF-Y to DNA, accelerates the ubiquitin-independent degradation of the NF-YA subunit, and reduces the transcriptional activity of NF-Y. As an antimitotic agent, NFYi5 decreases the mRNA levels of NF-Y target genes without affecting the expression of housekeeping genes, and inhibits cell proliferation. NFYi5 can be used in the research of tissue fibrosis^[1].

NFYi5 (0.01-100 μM; 18 h) inhibits NF-Y-dependent reporter gene activity in a dose-dependent manner, with an IC₅₀ of 19.95 μM in human cardiac fibroblasts and 12.73 μM in rat cardiac fibroblasts, while exerting no effect on non-NF-Y promoter activity^[1].
NFYi5 (10-20 μM; 18 h) selectively reduces the mRNA levels of NF-Y target genes in human and rat cardiac fibroblasts without affecting the expression of housekeeping genes^[1].
NFYi5 (5-20 μM; 24-48 h) inhibits the proliferation of human and rat cardiac fibroblasts in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

350.42

C₁₉H₂₂N₆O

925644-66-2

CC1=C2C(C(C)=NC(NC3=NCN(CN3)CC4=CC=CO4)=N2)=CC(C)=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ebrahimighaei R, et al. In Silico Discovery and Characterization of a Novel Nuclear Transcription Factor-Y (NF-Y) Inhibitor with Antimitogenic Properties. J Med Chem. Published online March 19, 2026.