NFYi5
NFYi5 is a nuclear transcription factor-Y (NF-Y) inhibitor. NFYi5 disrupts the binding of NF-Y to DNA, accelerates the ubiquitin-independent degradation of the NF-YA subunit, and reduces the transcriptional activity of NF-Y. As an antimitotic agent, NFYi5 decreases the mRNA levels of NF-Y target genes without affecting the expression of housekeeping genes, and inhibits cell proliferation. NFYi5 can be used in the research of tissue fibrosis.
For research use only. We do not sell to patients.
- CAS No.: 925644-66-2
- Formula: C19H22N6O
- Molecular Weight:350.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Biological Activity
NFYi5 (0.01-100 μM; 18 h) inhibits NF-Y-dependent reporter gene activity in a dose-dependent manner, with an IC50 of 19.95 μM in human cardiac fibroblasts and 12.73 μM in rat cardiac fibroblasts, while exerting no effect on non-NF-Y promoter activity[1].
NFYi5 (10-20 μM; 18 h) selectively reduces the mRNA levels of NF-Y target genes in human and rat cardiac fibroblasts without affecting the expression of housekeeping genes[1].
NFYi5 (5-20 μM; 24-48 h) inhibits the proliferation of human and rat cardiac fibroblasts in a dose-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human cardiac fibroblasts, rat cardiac fibroblasts
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Concentration:10, 20 μM
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Incubation Time:18 h
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Result:Reduced mRNA levels of NF-Y target genes CCNA2 and CCNB1 in human cardiac fibroblasts without altering GAPDH levels.
Dose-dependently reduced mRNA levels of NF-Y target genes CCNA2, CCNB1, ECT2, and DDX11 in rat cardiac fibroblasts, while leaving housekeeping gene mRNA levels (TBP, 36B4, GAPDH, UBC) unchanged.
Chemical Information
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CAS No. 925644-66-2
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Molecular Weight 350.42
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Formula C19H22N6O
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SMILES
CC1=C2C(C(C)=NC(NC3=NCN(CN3)CC4=CC=CO4)=N2)=CC(C)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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