2. Metabolic Enzyme/Protease
  3. ATGL
  4. NG-497

NG-497 is a selective human adipose triglyceride lipase (ATGL) inhibitor that targets the enzymatically active patatin-like domain of human ATGL. NG-497 has potential value for tumor research.

For research use only. We do not sell to patients.

CAS No. : 2598242-66-9

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Customer Review

Based on 3 publication(s) in Google Scholar

NG-497 Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
Flow Cytometry
IF
RT-PCR
WB

    NG-497 purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 3.  [Abstract]

    HMC3 cells were treated with NG-497 at various concentrations (0, 15.625, 31.25, 62.5, 125, 250, 500, and 1000 nM) for 24 h, and cell viability was then assessed using the CCK-8 assay.

    NG-497 purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 3.  [Abstract]

    Representative flow cytometry histograms showing the mean fluorescence intensity (MFI) of BODIPY in LPS-stimulated HMC3 cells treated with or without 500 nM NG-497.

    NG-497 purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 3.  [Abstract]

    Representative immunofluorescence images of BODIPY staining in LPS-stimulated HMC3 cells following treatment with or without NG-497 (500 nM).

    NG-497 purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 3.  [Abstract]

    qPCR analysis of the expression of pro-inflammatory factors (TNF-α, IL-6, IL-1β, and CCL-2) in LPS-stimulated HMC3 cells treated with or without NG-497 (125, 250, and 500 nM).

    NG-497 purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 3.  [Abstract]

    Western blot analysis of MTNR1A and MTNR1B expression in HMC3 cells following LPS stimulation and NG-497 pretreatment (125, 250, and 500 nM).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    NG-497 is a selective human adipose triglyceride lipase (ATGL) inhibitor that targets the enzymatically active patatin-like domain of human ATGL. NG-497 has potential value for tumor research[1].

    In Vitro

    NG-497 selectively inactivates human and nonhuman primate ATGL but not structurally and functionally related lipid hydrolases and abolishes human adipocytes in a dose-dependent and reversible manner Lipolysis[1].
    NG-497 (40 μM, 80 min) reduces lipolysis-dependent respiration in HepG2 cells without impairing mitochondrial function through off-target effects[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    NG-497 Related Antibodies
    Molecular Weight

    315.36

    Formula

    C18H21NO4
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(C1=CC(OC)=CC(C2=CC=C(OCC)C=C2)=N1)OC(C)C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 70 mg/mL (221.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1710 mL 15.8549 mL 31.7098 mL
    5 mM 0.6342 mL 3.1710 mL 6.3420 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1710 mL 15.8549 mL 31.7098 mL 79.2745 mL
    5 mM 0.6342 mL 3.1710 mL 6.3420 mL 15.8549 mL
    10 mM 0.3171 mL 1.5855 mL 3.1710 mL 7.9274 mL
    15 mM 0.2114 mL 1.0570 mL 2.1140 mL 5.2850 mL
    20 mM 0.1585 mL 0.7927 mL 1.5855 mL 3.9637 mL
    25 mM 0.1268 mL 0.6342 mL 1.2684 mL 3.1710 mL
    30 mM 0.1057 mL 0.5285 mL 1.0570 mL 2.6425 mL
    40 mM 0.0793 mL 0.3964 mL 0.7927 mL 1.9819 mL
    50 mM 0.0634 mL 0.3171 mL 0.6342 mL 1.5855 mL
    60 mM 0.0528 mL 0.2642 mL 0.5285 mL 1.3212 mL
    80 mM 0.0396 mL 0.1982 mL 0.3964 mL 0.9909 mL
    100 mM 0.0317 mL 0.1585 mL 0.3171 mL 0.7927 mL
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    NG-497 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    NG-4972598242-66-9NG497NG 497ATGLAdipose triglyceride lipasehumanpatatintumorInhibitorinhibitorinhibit

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