1. Epigenetics
  2. Histone Acetyltransferase
  3. NiCur

NiCur 

Cat. No.: HY-139149
Handling Instructions

NiCur is a potent and selective CBP HAT inhibitor with an IC50 value of 0.35 μΜ. NiCur can be used for performing mechanistic studies without affecting the expression of target proteins.

For research use only. We do not sell to patients.

NiCur Chemical Structure

NiCur Chemical Structure

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Description

NiCur is a potent and selective CBP HAT inhibitor with an IC50 value of 0.35 μΜ. NiCur can be used for performing mechanistic studies without affecting the expression of target proteins[1].

IC50 & Target

CBP

0.35 μM (IC50)

In Vitro

NiCur (0.5~1 μM; U2OS cells) reduces the Dox-induced p53K382ac, p53S15p, and p53 levels in a dose-dependent manner[1].
NiCur (1.5 μM) reduces the level of H3K27ac. NiCur (1.5 μM; U2OS cells) restores cellular proliferation. NiCur (Intestinal epithelial cells) down-regulates Dox-mediated p53 activation without affecting the levels of H2A.X S139p. NiCur can modulate the gene regulatory switch for reprogramming chromatin landscape. NiCur blocks CBP HAT activity[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: U2OS cells
Concentration: 0.5~1 μM
Incubation Time:
Result: Reduced the Dox-induced p53K382ac, p53S15p, and p53 levels in a dose-dependent manner.
Molecular Weight

324.38

Formula

C₂₂H₁₆N₂O

SMILES

N#CC1=CC=CC(/C=C2C(/C(CCC/2)=C/C3=CC(C#N)=CC=C3)=O)=C1.[(E)].[(E)]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (154.14 mM; ultrasonic and warming and heat to 80°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0828 mL 15.4140 mL 30.8280 mL
5 mM 0.6166 mL 3.0828 mL 6.1656 mL
10 mM 0.3083 mL 1.5414 mL 3.0828 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.67 mg/mL (5.15 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 1.67 mg/mL (5.15 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
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Keywords:

NiCurHistone AcetyltransferaseHATsHATU2OSintestinalepithelialp53K382acp53S15pand p53Inhibitorinhibitorinhibit

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NiCur
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HY-139149
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