NFAT Inhibitor-1
Based on 15 publication(s) in Google Scholar
NFAT Inhibitor (VIVIT peptide) is an inhibitor of nuclear factor of activated Tcells (NFAT) that selectively inhibits calcineurin-mediated dephosphorylation of NFAT.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 249537-73-3
- Formula: C75H118N20O22S
- Molecular Weight:1683.93
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) NFAT Inhibitor-1
More- Immunity. 2024 Jun 11;57(6):1289-1305.e9. [Abstract]
- Arthritis Rheumatol. 2019 Aug;71(8):1252-1264. [Abstract]
- J Immunother Cancer. 2021 Jul;9(7):e002840. [Abstract]
- Free Radic Biol Med. 2025 Aug 8:240:15-28. [Abstract]
- Biomed Pharmacother. 2023 May:161:114480. [Abstract]
- Cell Rep. 2026 Mar 13;45(3):117102. [Abstract]
- Sci Signal. 2018 Oct 16;11(552). pii: eaao5150. [Abstract]
- Cell Biosci. 2022 May 26;12(1):70. [Abstract]
- Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
- Int J Mol Sci. 2023 Feb 16;24(4):4022. [Abstract]
- Am J Physiol Lung Cell Mol Physiol. 2024 Jan 1;326(1):L39-L51. [Abstract]
- Exp Ther Med. 2023 Mar 6;25(4):172. [Abstract]
- Exp Ther Med. 2022 Nov 8;24(6):750. [Abstract]
- Tohoku J Exp Med. 2021 Apr;253(4):249-259. [Abstract]
- Northwestern University. 2026.
Customer Validation & Images
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WB
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IF
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IF
Biological Activity
NFAT Inhibitor-1 treatment significantly inhibits nuclear translocation of NFATc1 for 24 hours. Long-term treatment with NFAT Inhibitor-1 significantly inhibits the cytoplasmic levels of cathepsin K, TRAP, and MMP-9[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human peripheral blood CD14+ monocytes
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Concentration:10 μM
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Incubation Time:24 hours or 21 days
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Result:Short-term treatment with 10 μM, significantly inhibited nuclear translocation of NFATc1. Long-term treatment, significantly inhibited the cytoplasmic levels of cathepsin K, TRAP, and MMP-9.
Chemical Information
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CAS No. 249537-73-3
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Appearance Solid
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Molecular Weight 1683.93
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Formula C75H118N20O22S
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Color White to off-white
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Synonyms
VIVIT peptide
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Sequence
Met-Ala-Gly-Pro-His-Pro-Val-Ile-Val-Ile-Thr-Gly-Pro-His-Glu-Glu
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Sequence Shortening
MAGPHPVIVITGPHEE
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (15)
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Journal Impact Factor
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Most Recent
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Immunity
Metabolic regulator LKB1 controls adipose tissue ILC2 PD-1 expression and mitochondrial homeostasis to prevent insulin resistance. [Abstract]2024 Jun 11;57(6):1289-1305.e9. PMID: 38772366 -
Arthritis Rheumatol
Activation of the Peroxisome Proliferator-Activated Receptor γ Coactivator 1β/NFATc1 Pathway in Circulating Osteoclast Precursors Associated With Bone Destruction in Rheumatoid Arthritis. [Abstract]2019 Aug;71(8):1252-1264. PMID: 30802366 -
J Immunother Cancer
Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation. [Abstract]2021 Jul;9(7):e002840. PMID: 34326168 -
Free Radic Biol Med
Calcineurin/NFAT1/Smad2 signaling regulates microglial autophagy that contributes to neuroinflammation and cognitive deficit in mice with sepsis-associated encephalopathy. [Abstract]2025 Aug 8:240:15-28. PMID: 40784585 -
Biomed Pharmacother
Interruption of TRPC6-NFATC1 signaling inhibits NADPH oxidase 4 and VSMCs phenotypic switch in intracranial aneurysm. [Abstract]2023 May:161:114480. PMID: 37002575 -
Cell Rep
TCR-NF-κB signaling activates the SENP1-NR4A1 axis to fine-tune effector activation of CD8+ T cells. [Abstract]2026 Mar 13;45(3):117102. PMID: 41831233 -
Sci Signal
Decreased abundance of TRESK two-pore domain potassium channels in sensory neurons underlies the pain associated with bone metastasis. [Abstract]2018 Oct 16;11(552). pii: eaao5150. PMID: 30327410 -
Cell Biosci
2022 May 26;12(1):70. PMID: 35619122 -
Cell Prolif
WNT16b promotes the proliferation and self-renewal of human limbal epithelial stem/progenitor cells via activating the calcium/calcineurin A/NFATC2 pathway. [Abstract]2023 Oct;56(10):e13460. PMID: 36974338
NFAT Inhibitor-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
VIVIT (1 μM; 14 days) inhibits the protein expression of NFATC2 in hLESCs.
NFAT Inhibitor-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
VIVIT (1 μM; 14 days) inhibits the effect of WNT16b on cell proliferation in hLESCs.
NFAT Inhibitor-1 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
VIVIT (1 μM; 14 days) significantly inhibits the expression of WNT16b-promoting cell proliferation marker Ki67 and the putative hLESC marker ΔNp63α in hLESCs.
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Int J Mol Sci
Gsmtx4 Alleviated Osteoarthritis through Piezo1/Calcineurin/NFAT1 Signaling Axis under Excessive Mechanical Strain. [Abstract]2023 Feb 16;24(4):4022. PMID: 36835440 -
Am J Physiol Lung Cell Mol Physiol
Genetic deficiency of the transcription factor NFAT1 confers protection against fibrogenic responses independent of immune influx. [Abstract]2024 Jan 1;326(1):L39-L51. PMID: 37933452 -
Exp Ther Med
4'‑O‑methylbavachalcone inhibits succinate induced cardiomyocyte hypertrophy via the NFATc4 pathway. [Abstract]2023 Mar 6;25(4):172. PMID: 37006873 -
Exp Ther Med
PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells. [Abstract]2022 Nov 8;24(6):750. PMID: 36561964 -
Tohoku J Exp Med
Fusobacterium nucleatum Promotes Cisplatin-Resistance and Migration of Oral Squamous Carcinoma Cells by Up-Regulating Wnt5a-Mediated NFATc3 Expression. [Abstract]2021 Apr;253(4):249-259. PMID: 33840648 -
Solvent & Solubility
H2O : 100 mg/mL (59.38 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (29.69 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (283 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Aramburu J, et al. Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Science. 1999 Sep 24;285(5436):2129-33. [Content Brief]
[2]. Ma JD, et al. Activation of the Peroxisome Proliferator-Activated Receptor γ Coactivator 1β/NFATc1 Pathway in Circulating Osteoclast Precursors Associated With Bone Destruction in Rheumatoid Arthritis. Arthritis Rheumatol. 2019 Aug;71(8):1252-1264. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.5938 mL | 2.9692 mL | 5.9385 mL | 14.8462 mL |
| 5 mM | 0.1188 mL | 0.5938 mL | 1.1877 mL | 2.9692 mL | |
| 10 mM | 0.0594 mL | 0.2969 mL | 0.5938 mL | 1.4846 mL | |
| 15 mM | 0.0396 mL | 0.1979 mL | 0.3959 mL | 0.9897 mL | |
| 20 mM | 0.0297 mL | 0.1485 mL | 0.2969 mL | 0.7423 mL | |
| 25 mM | 0.0238 mL | 0.1188 mL | 0.2375 mL | 0.5938 mL | |
| 30 mM | 0.0198 mL | 0.0990 mL | 0.1979 mL | 0.4949 mL | |
| 40 mM | 0.0148 mL | 0.0742 mL | 0.1485 mL | 0.3712 mL | |
| 50 mM | 0.0119 mL | 0.0594 mL | 0.1188 mL | 0.2969 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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