2. Apoptosis
  3. Apoptosis
  4. NPD15261

NPD15261 (MI102) is a highly selective MYCN inhibitor. NPD15261 reduces the mRNA and protein levels of MYCN in liver cancer cells, inhibits cell proliferation and colony formation, and induces Apoptosis simultaneously. NPD15261 can be used in liver cancer research.

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NPD15261

NPD15261 Chemical Structure

CAS No. : 895901-42-5

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NPD15261 Related Antibodies

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Description

NPD15261 (MI102) is a highly selective MYCN inhibitor. NPD15261 reduces the mRNA and protein levels of MYCN in liver cancer cells, inhibits cell proliferation and colony formation, and induces Apoptosis simultaneously. NPD15261 can be used in liver cancer research[1].

In Vitro

NPD15261 (0-10 μM; 2 h) inhibits basal and HGF-induced phosphorylation of MET at Y1234/Y1235 in JHH7 cells with an IC50 of 0.77 μM, and also suppresses HGF-induced MET phosphorylation at Y1349[1].
NPD15261 (0-10 μM) dose-dependently inhibits the expression of MYCN mRNA and protein in JHH7 cells, and this effect can be partially reversed by stimulation with 20 ng/mL HGF[1].
NPD15261 (0-5 μM; 4 days) selectively reduces the viability of JHH7 cells and the survival rate of JHH7 spheroids at the concentration of 5 μM, with no effect on normal Hc cells[1].
NPD15261 (2.5-10 μM; 0-12 days) inhibits colony formation of JHH7 cells and exerts a dose-dependent inhibitory effect on the proliferation of JHH7 cells[1].
NPD15261 (2.5 μM; 24 h) induces apoptosis in JHH7 cells, while exhibiting extremely low cytotoxicity against normal Hc cells[1].
NPD15261 (5-10 μM; 6 h) downregulates MYCN-related oncogenic pathways (including MYC_Targets_V2, MYC_Targets_V1 and E2F_Targets), and selectively inhibits MYCN target genes in JHH7 cells after 6 hours of treatment, with no effect on normal Hc cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NPD15261 Related Antibodies

Western Blot Analysis[1]

Cell Line: JHH7 cells
Concentration: 0, 0.5, 1, 2.5, 5, 10 μM , with or without HGF (10 ng/mL)
Incubation Time: 2 h
Result: Inhibited the phosphorylation of MET at Y1234/Y1235 as well as HGF-induced phosphorylation at Y1234/Y1235 and Y1349 in a dose-dependent manner.
Inhibited MET phosphorylation at Y1234/Y1235 by approximately 83% at 1 μM, with an estimated IC50 value
of 0.77 μM.

Cell Viability Assay[1]

Cell Line: human hepatocellular carcinoma JHH7 cells, normal hepatic Hc cells
Concentration: 0, 0.2, 1, 5 μM
Incubation Time: 4 days
Result: Markedly reduced JHH7 cell viability at 5 μM.
Showed no observable effects on normal hepatic Hc cells.

Apoptosis Analysis[1]

Cell Line: human hepatocellular carcinoma JHH7 cells, normal hepatic Hc cells
Concentration: 2.5 μM
Incubation Time: 24 h
Result: Induced apoptosis in JHH7 cells.
Showed no significant cytotoxicity to normal hepatic Hc cells.
Molecular Weight

476.56

Formula

C28H32N2O5
CAS No.
SMILES

CNC1CCC2=C(C(OC)=C(OC)C(OC)=C2)C(C1=C3)=CC=C(NCC4=C(OC)C=CC=C4)C3=O
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Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.
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References
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NPD15261 Related Classifications

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Keywords:

NPD15261895901-42-5MI102NPD 15261NPD-15261MI 102MI-102ApoptosisMYCN inhibitorJHH7 cellsliver cancer
Inhibitorinhibitorinhibit

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