DHBS
Based on 1 Customer Validation
DHBS (3, 5-dichloro-2-hydroxybenzenesulfonate) is used in conjunction with 4-aminoantipyrine (4-AAP) (HY-W100990) and hydrogen peroxide (H2O2) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 54970-72-8
- Formula: C6H3Cl2NaO4S
- Molecular Weight:265.05
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DHBS (750 μM, 5 min) significantly enhances nitroblue tetrazolium (NBT) induced O2 reduction in vitro[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 54970-72-8
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Appearance Solid
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Molecular Weight 265.05
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Formula C6H3Cl2NaO4S
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Color White to off-white
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SMILES
O=S(C1=CC(Cl)=CC(Cl)=C1O)(O[Na])=O
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Synonyms
DCHBS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 100 mg/mL (377.29 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Tarasek D, et al. In vitro oxidation of hispidin and gallic acid by horseradish peroxidase[J]. J Biomol Struct Dyn. 2023 Apr;41(6):2321-2325. [Content Brief]
[2]. Aizenman E. Modulation of N-methyl-D-aspartate receptors by hydroxyl radicals in rat cortical neurons in vitro[J]. Neurosci Lett. 1995 Apr 7;189(1):57-9. [Content Brief]
[3]. Colliss JS, et al. The analysis of serum urate utilizing immobilized uricase[J]. Appl Biochem Biotechnol. 1981 Mar;6(1):15-24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.7729 mL | 18.8644 mL | 37.7287 mL | 94.3218 mL |
| 5 mM | 0.7546 mL | 3.7729 mL | 7.5457 mL | 18.8644 mL | |
| 10 mM | 0.3773 mL | 1.8864 mL | 3.7729 mL | 9.4322 mL | |
| 15 mM | 0.2515 mL | 1.2576 mL | 2.5152 mL | 6.2881 mL | |
| 20 mM | 0.1886 mL | 0.9432 mL | 1.8864 mL | 4.7161 mL | |
| 25 mM | 0.1509 mL | 0.7546 mL | 1.5091 mL | 3.7729 mL | |
| 30 mM | 0.1258 mL | 0.6288 mL | 1.2576 mL | 3.1441 mL | |
| 40 mM | 0.0943 mL | 0.4716 mL | 0.9432 mL | 2.3580 mL | |
| 50 mM | 0.0755 mL | 0.3773 mL | 0.7546 mL | 1.8864 mL | |
| 60 mM | 0.0629 mL | 0.3144 mL | 0.6288 mL | 1.5720 mL | |
| 80 mM | 0.0472 mL | 0.2358 mL | 0.4716 mL | 1.1790 mL | |
| 100 mM | 0.0377 mL | 0.1886 mL | 0.3773 mL | 0.9432 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DHBS Related Classifications
HY-15914 Related Classifications
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