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Cat. No.: HY-16934

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ML352

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Based on 1 Customer Validation

ML352 is a noncompetitive inhibitor of the presynaptic choline transporter (CHT) with K_i values of 92 and 166 nM for HEK293 cells expressing human CHT and mouse forebrain synaptosomes, respectively.

For research use only. We do not sell to patients.

Purity: 99.78%
CAS No.: 1649450-12-3
Formula: C₂₁H₂₉N₃O₄
Molecular Weight:387.47

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

ML352 Chemical Structure

CAS No.: 1649450-12-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Cholinesterase (ChE) Isoforms :

AChE
BChE

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.09 μM Compound: 10m, ML352	Inhibition of human CHT Leu530Ala and Val531Ala mutant expressed in HEK293 cells assessed as remaining transporter activity in presence of 10 uM [3H]choline by [3H]choline uptake assay Inhibition of human CHT Leu530Ala and Val531Ala mutant expressed in HEK293 cells assessed as remaining transporter activity in presence of 10 uM [3H]choline by [3H]choline uptake assay	[PMID: 25801932]
HEK293	IC₅₀	0.27 μM Compound: 10m, ML352	Inhibition of human CHT Leu530Ala and Val531Ala mutant expressed in HEK293 cells assessed as remaining transporter activity in presence of 100 nM [3H]choline by [3H]choline uptake assay Inhibition of human CHT Leu530Ala and Val531Ala mutant expressed in HEK293 cells assessed as remaining transporter activity in presence of 100 nM [3H]choline by [3H]choline uptake assay	[PMID: 25801932]
HEK293	IC₅₀	72 nM Compound: 2; ML352; VU0476201	Inhibition of [3H]choline uptake at human choline transporter expressed in HEK293 cells preincubated for 15 mins followed by [3H]choline addition measured after 10 to 15 mins by scintillation proximity assay Inhibition of [3H]choline uptake at human choline transporter expressed in HEK293 cells preincubated for 15 mins followed by [3H]choline addition measured after 10 to 15 mins by scintillation proximity assay	[PMID: 27575469]

Chemical Information

CAS No. 1649450-12-3
Appearance Solid-Liquid Mixture
Molecular Weight 387.47
Formula C₂₁H₂₉N₃O₄
Color White to light yellow
SMILES

CC(C)C1=NOC(CNC(C2=CC(OC3CCN(C)CC3)=C(OC)C=C2)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (645.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5808 mL

12.9042 mL

25.8084 mL

5 mM

0.5162 mL

2.5808 mL

5.1617 mL

10 mM

0.2581 mL

1.2904 mL

2.5808 mL

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ennis EA, Wright J, Retzlaff CL, McManus OB, Lin Z, Huang X, et al. Identification and characterization of ML352: a novel, noncompetitive inhibitor of the presynaptic choline transporter. ACS Chem Neurosci. 2015 Mar 18;6(3):417-27. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5808 mL	12.9042 mL	25.8084 mL	64.5211 mL
	5 mM	0.5162 mL	2.5808 mL	5.1617 mL	12.9042 mL
	10 mM	0.2581 mL	1.2904 mL	2.5808 mL	6.4521 mL
	15 mM	0.1721 mL	0.8603 mL	1.7206 mL	4.3014 mL
	20 mM	0.1290 mL	0.6452 mL	1.2904 mL	3.2261 mL
	25 mM	0.1032 mL	0.5162 mL	1.0323 mL	2.5808 mL
	30 mM	0.0860 mL	0.4301 mL	0.8603 mL	2.1507 mL
	40 mM	0.0645 mL	0.3226 mL	0.6452 mL	1.6130 mL
	50 mM	0.0516 mL	0.2581 mL	0.5162 mL	1.2904 mL
	60 mM	0.0430 mL	0.2151 mL	0.4301 mL	1.0754 mL
	80 mM	0.0323 mL	0.1613 mL	0.3226 mL	0.8065 mL
	100 mM	0.0258 mL	0.1290 mL	0.2581 mL	0.6452 mL

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100 mg

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Biological Activity
- Description
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

ML352

Rivastigmine

VU0364572 TFA

Arecoline hydrobromide

Nitenpyram

Galanthamine hydrobromide

(±)-Vesamicol hydrochloride

Flavoxate hydrochloride

Rivastigmine (Standard)

Flavoxate hydrochloride (Standard)

Nitenpyram (Standard)

Arecoline hydrobromide (Standard)

Galanthamine hydrobromide (Standard)

All Cholinesterase (ChE) Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

ML352

Rivastigmine

VU0364572 TFA

Arecoline hydrobromide

Nitenpyram

Galanthamine hydrobromide

(±)-Vesamicol hydrochloride

Flavoxate hydrochloride

Rivastigmine (Standard)

Flavoxate hydrochloride (Standard)

Nitenpyram (Standard)

Arecoline hydrobromide (Standard)

Galanthamine hydrobromide (Standard)

All Cholinesterase (ChE) Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ML352 Related Classifications

HY-16934 Related Classifications

Bioz