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  3. Hapten
  4. O-Phthalimide-C5-acid

6-N-Phthalimidoy hexanoic acid (compound FH) is a hapten with a carboxyl group at the end of its spacer arm, suitable for reacting with free amine groups of proteins. 6-N-Phthalimidoy hexanoic acid can be combined with carrier proteins and used in antigen design.

For research use only. We do not sell to patients.

O-Phthalimide-C5-acid Chemical Structure

O-Phthalimide-C5-acid Chemical Structure

CAS No. : 4443-26-9

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Description

6-N-Phthalimidoy hexanoic acid (compound FH) is a hapten with a carboxyl group at the end of its spacer arm, suitable for reacting with free amine groups of proteins. 6-N-Phthalimidoy hexanoic acid can be combined with carrier proteins and used in antigen design[1].

Molecular Weight

261.27

Formula

C14H15NO4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2C=CC=C1)N(CCCCCC(O)=O)C2=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (382.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8275 mL 19.1373 mL 38.2746 mL
5 mM 0.7655 mL 3.8275 mL 7.6549 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8275 mL 19.1373 mL 38.2746 mL 95.6865 mL
5 mM 0.7655 mL 3.8275 mL 7.6549 mL 19.1373 mL
10 mM 0.3827 mL 1.9137 mL 3.8275 mL 9.5686 mL
15 mM 0.2552 mL 1.2758 mL 2.5516 mL 6.3791 mL
20 mM 0.1914 mL 0.9569 mL 1.9137 mL 4.7843 mL
25 mM 0.1531 mL 0.7655 mL 1.5310 mL 3.8275 mL
30 mM 0.1276 mL 0.6379 mL 1.2758 mL 3.1895 mL
40 mM 0.0957 mL 0.4784 mL 0.9569 mL 2.3922 mL
50 mM 0.0765 mL 0.3827 mL 0.7655 mL 1.9137 mL
60 mM 0.0638 mL 0.3190 mL 0.6379 mL 1.5948 mL
80 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1961 mL
100 mM 0.0383 mL 0.1914 mL 0.3827 mL 0.9569 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
O-Phthalimide-C5-acid
Cat. No.:
HY-W028210
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