Octanal
Based on 1 publication(s) in Google Scholar
Octanal is a fragrant aldehyde with antioxidant and antibacterial activity. Its antifungal effect against G. citri-aurantii may be related to the disruption of cell membrane integrity and the leakage of cellular components. Additionally, Octanal exhibits cytotoxicity towards HeLa cells, with an IC50 value of 3.5 μg/mL.
For research use only. We do not sell to patients.
- Purity : 98.20%
- CAS No.: 124-13-0
- Formula: C8H16O
- Molecular Weight:128.22
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-N0098
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HY-N8015R
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HY-N0098R
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HY-N8016
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HY-N8016R
Nonanal (Standard)Saturated Fatty Aldehyde -
HY-W013627
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HY-Y1356
2,4-DimethoxybenzaldehydeTumor Initiator -
HY-Y0543
5-MethylfurfuralBiochemical Reagent -
HY-W020678
2-MethylbutanalBiomarker -
HY-Y0543R
5-Methylfurfural (Standard)Biochemical Reagent
Publications Citing Use of MedChemExpress (MCE) Octanal
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Customer Validation & Images
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Histological Imaging/Staining
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WB
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IF
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RT-PCR
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In Vivo Efficacy Study
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Octanal (0-8 μL/mL, 120 min) increases the extracellular conductivity, enhances the membrane permeability, and decreases the lipid content in G. citri-aurantii mycelium[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 124-13-0
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Appearance Liquid (Density: 0.821 g/cm3)
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Molecular Weight 128.22
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Formula C8H16O
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Color Colorless to light yellow
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SMILES
CCCCCCCC=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Sci Rep
NHE1 in macrophages promotes octanal/Olfr2-induced atherosclerosis via calcium-dependent ROS and NLRP3 inflammasome activation. [Abstract]2025 Dec 29;15(1):44695. PMID: 41461716
Octanal purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 29;15(1):44695. [Abstract]
These sections underwent hematoxylin and eosin (H&E) staining, oil red O staining and Masson’s trichrome staining treated with Octanal (Oct) (10 μg/g, i.p.).
Octanal purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 29;15(1):44695. [Abstract]
In vitro experiments demonstrate the expression levels and statistical analysis of NHE1 protein in RAW264.7 cells subjected to Octanal (Oct) (0, 5, 10, 20, 40 μM) treatment for varying durations or at different concentrations.
Octanal purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 29;15(1):44695. [Abstract]
Fluorescence images depicting pH alterations in RAW264.7 cells exposed to different concentrations of octanal, visualized using a BCECF-AM probe treated with Octanal (Oct) (0., 5, 10, 20, 40 μM).
Octanal purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 29;15(1):44695. [Abstract]
qPCR analysis of NLRP3, IL-1β and IL-6 mRNA expression treated with Octanal (Oct) (20 μM).
Octanal purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Dec 29;15(1):44695. [Abstract]
Images of atherosclerotic plaque in the aortic arch of NHE+/+ ApoE−/− and NHE+/−ApoE−/− mice with or without high-fat diet (HFD) or plus Octanal (OCT) (10 μg/g, i.p.).
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (779.91 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (19.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (19.50 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (605 KB)
- English - EN (605 KB)
- Français - FR (605 KB)
- Deutsch - DE (605 KB)
- Norwegian - NO (605 KB)
- Español - ES (605 KB)
- Swedish - SV (605 KB)
- Italian - IT (605 KB)
- Korean - KR (605 KB)
- Portuguese - PT (605 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.7991 mL | 38.9955 mL | 77.9910 mL | 194.9774 mL |
| 5 mM | 1.5598 mL | 7.7991 mL | 15.5982 mL | 38.9955 mL | |
| 10 mM | 0.7799 mL | 3.8995 mL | 7.7991 mL | 19.4977 mL | |
| 15 mM | 0.5199 mL | 2.5997 mL | 5.1994 mL | 12.9985 mL | |
| 20 mM | 0.3900 mL | 1.9498 mL | 3.8995 mL | 9.7489 mL | |
| 25 mM | 0.3120 mL | 1.5598 mL | 3.1196 mL | 7.7991 mL | |
| 30 mM | 0.2600 mL | 1.2998 mL | 2.5997 mL | 6.4992 mL | |
| 40 mM | 0.1950 mL | 0.9749 mL | 1.9498 mL | 4.8744 mL | |
| 50 mM | 0.1560 mL | 0.7799 mL | 1.5598 mL | 3.8995 mL | |
| 60 mM | 0.1300 mL | 0.6499 mL | 1.2998 mL | 3.2496 mL | |
| 80 mM | 0.0975 mL | 0.4874 mL | 0.9749 mL | 2.4372 mL | |
| 100 mM | 0.0780 mL | 0.3900 mL | 0.7799 mL | 1.9498 mL |
Octanal Related Classifications
HY-N8015 Related Classifications
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