2. Cell Cycle/DNA Damage Epigenetics Apoptosis
  3. PARP Apoptosis
  4. OUL312

OUL312 is a potent PARP10 inhibitor (IC50 = 20 nM), showing more than 75-fold greater selectivity for PARP15 (IC50 = 1500 nM). OUL312 is also at least 18-fold higher than all other human enzymes in the family. OUL312 has a good ADME profile, is able to enter cells, is not toxic, and effectively rescues human cells from PARP10-induced apoptosis at sub-micromolar concentrations. OUL312 can be used for the study of cervical cancer.

For research use only. We do not sell to patients.

OUL312

OUL312 Chemical Structure

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
25 mg In-stock
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100 mg In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

OUL312 Related Antibodies

Top Publications Citing Use of Products

View All PARP Isoform Specific Products:

View All Isoforms
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

OUL312 is a potent PARP10 inhibitor (IC50 = 20 nM), showing more than 75-fold greater selectivity for PARP15 (IC50 = 1500 nM). OUL312 is also at least 18-fold higher than all other human enzymes in the family. OUL312 has a good ADME profile, is able to enter cells, is not toxic, and effectively rescues human cells from PARP10-induced apoptosis at sub-micromolar concentrations. OUL312 can be used for the study of cervical cancer[1].

IC50 & Target

PARP10

 

In Vitro

OUL312 (Compound 15) (0.05-10 μM, 10-12 days) shows no significant cytotoxicity in HeLa Flp-In TRex cells and could rescue Doxycycline (HY-N0565)-induced HeLa Flp-In TRex cells in a concentration-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

OUL312 Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: HeLa Flp-In T-Rex cells
Concentration: 10 μM
Incubation Time: 10-12 days
Result: Showed no significant cytotoxicity.

Cell Proliferation Assay[1]

Cell Line: Doxycycline-induced HeLa Flp-In T-Rex cells
Concentration: 0.05 μM, 0.1 μM, 0.5 μM, 1 μM, 10 μM
Incubation Time: 10-12 days
Result: Rescued cells in a concentration-dependent manner with apparent EC50 below 1 μM.
Molecular Weight

300.28

Formula

C16H13FN2O3
Appearance

Solid

Color

White to off-white

SMILES

O=C1NNC(C2=C1C=CC(OCCC3=CC=CC=C3F)=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (33.30 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3302 mL 16.6511 mL 33.3022 mL
5 mM 0.6660 mL 3.3302 mL 6.6605 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3302 mL 16.6511 mL 33.3023 mL 83.2556 mL
5 mM 0.6660 mL 3.3302 mL 6.6605 mL 16.6511 mL
10 mM 0.3330 mL 1.6651 mL 3.3302 mL 8.3256 mL
15 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5504 mL
20 mM 0.1665 mL 0.8326 mL 1.6651 mL 4.1628 mL
25 mM 0.1332 mL 0.6660 mL 1.3321 mL 3.3302 mL
30 mM 0.1110 mL 0.5550 mL 1.1101 mL 2.7752 mL
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OUL312 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OUL312OUL 312OUL-312PARPApoptosispoly ADP ribose polymerase 2,3-Dihydrophthalazine-1,4-dioneADP-RibosylationInhibitorPARP10inhibitorinhibit

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