p-Aminophenyl phosphate
p-Aminophenyl phosphate is a substrate of alkaline phosphatase (Alkaline Phosphatase, ALP). p-Aminophenyl phosphate is catalytically hydrolyzed and dephosphorylated by ALP under alkaline conditions to generate highly electroactive pAP. p-Aminophenyl phosphate can be used in studies for real-time monitoring of ALP release from living cells.
For research use only. We do not sell to patients.
- CAS No.: 52331-30-3
- Formula: C6H8NO4P
- Molecular Weight:189.11
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
p-Aminophenyl phosphate (0.2-5 mM; 10 min) sodium shows cell line-dependent Km values in the adherent cell ALP release assay: 4.75 mM for Balb/c 3T3, 5.03 mM for A549, 10.19 mM for MCF-7, and 1.85 mM for Ht-29[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 52331-30-3
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Molecular Weight 189.11
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Formula C6H8NO4P
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SMILES
O=P(OC1=CC=C(C=C1)N)(O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Mesenchymal stromal/stem cell osteogenic differentiation
Mesenchymal stromal/stem cells can be induced toward an osteoblast-like lineage in vitro by culture in osteogenic medium containing dexamethasone, ascorbic acid or ascorbate-2-phosphate, and β-glycerophosphate; the differentiation process is commonly evaluated by alkaline phosphatase activity, osteogenic marker expression, collagenous matrix formation, and calcium-rich matrix mineralization. The main readouts are alkaline phosphatase activity as an early osteogenic marker and Alizarin Red S staining as a calcium-deposit readout for mineralized extracellular matrix; Alizarin Red S can be inspected microscopically or extracted and measured colorimetrically at 405 nm.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
p-Aminophenyl phosphate Related Classifications
HY-W554649 Related Classifications
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