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Cat. No.: HY-149003

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PARP1-IN-10

PARP1-IN-10 (compound 12c) is a no-cytotoxicity and potent PARP1 inhibitor with an IC₅₀ value of 50.62 nM in vitro. PARP1-IN-10 causes cell cycle arrest at G2/M phase and apoptosis, and enhances the cytotoxicity of temozolomide (TMZ) .

For research use only. We do not sell to patients.

CAS No.: 2494001-21-5
Formula: C₂₀H₂₃N₃O₅
Molecular Weight:385.41

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

PARP1-IN-10 Chemical Structure

CAS No.: 2494001-21-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

PARP1

50.62 nM (IC₅₀, ^[1])

All PARP Isoforms :

In Vitro

PARP1-IN-10 (compound 12c) (10 μM, 48 h) shows no cytotoxic effects against NCI-60 human tumor cell lines^[1].
PARP1-IN-10 inhibits MDA-MB-436 cell line with an IC₅₀ value of 3.73 μM^[1].
PARP1-IN-10 (1 and 3.73 μM, 48 h) causes cell cyle arrest at G2/M with dose-dependent manner^[1].
PARP1-IN-10 (0.5 μM, 48 h) shows antiprolifetative effect of temozolomide (TMZ) about 7 times (IC₅₀ = 3.64 μM) in A549 cell line compared to TMZ alone (IC₅₀=24.2 μM)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

NCI-60 human tumor cells
Concentration:

10 μM
Incubation Time:

48 hours
Result:

Showed no toxicity.

Cell Cycle Analysis^[1]

Cell Line:

MDA-MB-436 cells
Concentration:

0, 1, 3.73 μM
Incubation Time:

48 hours
Result:

Caused cell cycle arrest at G2/M and showed apoptotic effect in dose-dependent manner.

Apoptosis Analysis^[1]

Cell Line:

A549 human lung cancer cells
Concentration:

0, 0.5, 7.94 μM
Incubation Time:

48 hours
Result:

Potentiated the antiproliferative effect of temozolomide (TMZ) 7 times compared with TMZ alone.

Chemical Information

CAS No. 2494001-21-5
Molecular Weight 385.41
Formula C₂₀H₂₃N₃O₅
SMILES

O=C1/C(C(N(C)C(N1C)=O)=O)=C\C2=CC=C(C=C2)OCC(N3CCCCC3)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Essam Eldin A. Osman, et al. Design and synthesis of some barbituric and 1,3-dimethylbarbituric acid derivatives: A non-classical scaffold for potential PARP1 inhibitors. Bioorg Chem. 2020 Aug; 104: 104198.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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PARP1-IN-10

All PARP Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

PARP1-IN-10 Related Classifications

HY-149003 Related Classifications