2. Stem Cell/Wnt TGF-beta/Smad Immunology/Inflammation GPCR/G Protein
  3. TGF-beta/Smad CCR
  4. Pirfenidone-d5

Pirfenidone-d5  (Synonyms: AMR69-d5)

Cat. No.: HY-B0673S Purity: 99.48%
COA Handling Instructions

Molarity Calculator

Pirfenidone-d5 is a deuterium labeled Pirfenidone. Pirfenidone is an antifibrotic agent that attenuates CCL2 and CCL12 production in fibrocyte cells. Pirfenidone has growth-inhibitory effect and reduces TGF-β2 protein levels in human glioma cell lines. Pirfenidone also has anti-inflammatory activities[1][2][3].

For research use only. We do not sell to patients.

Pirfenidone-d<sub>5</sub> Chemical Structure

Pirfenidone-d5 Chemical Structure

CAS No. : 1020719-62-3

Size Price Stock Quantity
1 mg USD 170 In-stock
5 mg USD 510 In-stock
10 mg USD 820 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Pirfenidone-d5:

Pirfenidone-d5 Related Antibodies

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View All CCR Isoform Specific Products:

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CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Pirfenidone-d5 is a deuterium labeled Pirfenidone. Pirfenidone is an antifibrotic agent that attenuates CCL2 and CCL12 production in fibrocyte cells. Pirfenidone has growth-inhibitory effect and reduces TGF-β2 protein levels in human glioma cell lines. Pirfenidone also has anti-inflammatory activities[1][2][3].

IC50 & Target

TGF-β2[1][2]
CCL2 and CCL12[3]
Molecular Weight

190.25

Formula

C12H6D5NO
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1C=CC(C)=CN1C2=C([2H])C([2H])=C([2H])C([2H])=C2[2H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (525.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.2562 mL 26.2812 mL 52.5624 mL
5 mM 1.0512 mL 5.2562 mL 10.5125 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.25 mg/mL (32.85 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 6.25 mg/mL (32.85 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2562 mL 26.2812 mL 52.5624 mL 131.4060 mL
5 mM 1.0512 mL 5.2562 mL 10.5125 mL 26.2812 mL
10 mM 0.5256 mL 2.6281 mL 5.2562 mL 13.1406 mL
15 mM 0.3504 mL 1.7521 mL 3.5042 mL 8.7604 mL
20 mM 0.2628 mL 1.3141 mL 2.6281 mL 6.5703 mL
25 mM 0.2102 mL 1.0512 mL 2.1025 mL 5.2562 mL
30 mM 0.1752 mL 0.8760 mL 1.7521 mL 4.3802 mL
40 mM 0.1314 mL 0.6570 mL 1.3141 mL 3.2852 mL
50 mM 0.1051 mL 0.5256 mL 1.0512 mL 2.6281 mL
60 mM 0.0876 mL 0.4380 mL 0.8760 mL 2.1901 mL
80 mM 0.0657 mL 0.3285 mL 0.6570 mL 1.6426 mL
100 mM 0.0526 mL 0.2628 mL 0.5256 mL 1.3141 mL
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Pirfenidone-d5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pirfenidone-d51020719-62-3AMR69-d5TGF-beta/SmadCCRTransforming growth factor betaCC chemokine receptorPirfenidonedeuteriumlabeledantifibroticCCL2CCL12growth-inhibitoryTGF-β2anti-inflammatoryInhibitorinhibitorinhibit

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