Polyphosphate kinase
Based on 1 Customer Validation
Polyphosphate kinase (RsPPK; PPK) is a key enzyme in bacteria that catalyzes the reversible conversion between inorganic polyphosphate (polyP) and nucleoside phosphates (ATP/ADP/AMP, etc.). Polyphosphate kinase antagonizes the expression of virulence genes regulated by (p) ppGpp, PigR, MglA and SspA in Francisella tularensis. Polyphosphate kinase can be used in studies related to tularemia.
For research use only. We do not sell to patients.
- CAS No.: 9026-44-2
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Polyphosphate kinase exhibits strong ATP-dependent polyphosphate (polyP) synthesis activity in vitro in the presence of an ATP regeneration system, and it can phosphorylate ADP and GDP[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 9026-44-2
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SMILES
[Polyphosphate kinase]
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Synonyms
RsPPK; PPK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Purity & Documentation
References
[1]. Neville N, et al. Polyphosphate Kinase 2 (PPK2) Enzymes: Structure, Function, and Roles in Bacterial Physiology and Virulence. Int J Mol Sci. 2022;23(2):670. Published 2022 Jan 8. [Content Brief]
[2]. Rohlfing AE, et al. Polyphosphate Kinase Antagonizes Virulence Gene Expression in Francisella tularensis. J Bacteriol. 2018;200(3):e00460-17. Published 2018 Jan 10. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Polyphosphate kinase Related Classifications
HY-E70426 Related Classifications
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