Procumbenoside A
Procumbenoside A is a lignan glycoside discovered in Justicia procumbens. Procumbenoside A inhibits HIV-1 replication with an IC50 of 4.95 μM. Procumbenoside A exerts cytotoxic effects on various cancer cells. Procumbenoside A induces Cu (II)-mediated strand breaks in supercoiled plasmid DNA, generating the relaxed circular DNA form, while no linear form is detected. Procumbenoside A is used in studies related to liver cancer, colon adenocarcinoma, and HIV-1 infection.
For research use only. We do not sell to patients.
- CAS No.: 408358-51-0
- Formula: C31H32O15
- Molecular Weight:644.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
HIV-1 4.95 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| NIH3T3 | ED50 |
3.1 μg/mL
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Cytotoxic activity against 212 cells measured by MTT assay after 6 days of incubation.
Cytotoxic activity against 212 cells measured by MTT assay after 6 days of incubation.
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11908984 |
| Hep 3B2 | ED50 |
3.1 μg/mL
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Cytotoxic activity against human Hep 3B cells measured by MTT assay after 6 days of incubation.
Cytotoxic activity against human Hep 3B cells measured by MTT assay after 6 days of incubation.
|
11908984 |
| HepG2 | ED50 |
3.9 μg/mL
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Cytotoxic activity against human Hep G2 cells measured by MTT assay after 6 days of incubation.
Cytotoxic activity against human Hep G2 cells measured by MTT assay after 6 days of incubation.
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11908984 |
| HT-29 | ED50 |
6.7 μg/mL
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Cytotoxic activity against human HT-29 cells measured by MTT assay after 6 days of incubation.
Cytotoxic activity against human HT-29 cells measured by MTT assay after 6 days of incubation.
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11908984 |
| A549 | CC50 |
> 31.0 μM
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Cytotoxicity against human A549 lung epithelial cells assessed by MTS assay at 48 h after initial compound addition following 24 h incubation with procumbenoside A, with the CC50 value greater than 31.0 μM.
Cytotoxicity against human A549 lung epithelial cells assessed by MTS assay at 48 h after initial compound addition following 24 h incubation with procumbenoside A, with the CC50 value greater than 31.0 μM.
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31882050 |
In Vitro
Procumbenoside A (6 days) exerts significant cytotoxic effects on 212, Hep 3B, Hep G2, and HT-29 cells, with ED50 values of 3.1, 3.1, 3.9, and 6.7 µg/mL, respectively[1].
Procumbenoside A (0.3125-10 μM; 24-48 h) inhibits the replication of HIV-1 pseudovirus in A549 human lung epithelial cells, with an IC50 of 4.95 μM. It exhibits low cytotoxicity (CC50 > 31.0 μM) and a selectivity index greater than 6.2[2].
Procumbenoside A significantly induces Cu (II)-mediated concentration-dependent strand breaks in supercoiled pBR322 plasmid DNA, resulting in the formation of relaxed open-circle DNA[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549 human lung epithelial cells
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Concentration:0.3125, 0.625, 1.25, 2.5, 5 and 10 μM
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Incubation Time:24 h
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Result:Exhibited low cytotoxicity (CC50 > 31.0 μM).
Chemical Information
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CAS No. 408358-51-0
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Molecular Weight 644.58
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Formula C31H32O15
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SMILES
O=C1C2=C(C=3C(C(O[C@H]4[C@H](O[C@H]5[C@H](O)[C@@H](O)[C@@H](O)CO5)[C@](CO)(O)CO4)=C2CO1)=CC(OC)=C(OC)C3)C=6C=C7C(=CC6)OCO7
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Plasmid construction
Plasmid construction refers to the method of producing recombinant plasmid by connecting the target DNA molecule fragment with the DNA molecule of a specific carrier in Escherichia coli.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Day SH, et al. Potent cytotoxic lignans from Justicia procumbens and their effects on nitric oxide and tumor necrosis factor-alpha production in mouse macrophages. Journal of natural products. 2002 Mar;65(3):379-81. [Content Brief]
[2]. Xu XY, et al. Anti-HIV lignans from Justicia procumbens. Chinese journal of natural medicines. 2019 Dec;17(12):945-952. [Content Brief]
[3]. Lu YH, et al. DNA strand-scission by phloroglucinols and lignans from heartwood of Garcinia subelliptica Merr. and Justicia plants. Phytochemistry. 2008 Jan;69(1):225-33. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Procumbenoside A Related Classifications
HY-N21692 Related Classifications
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