Propargyl-PEG4-acid
Based on 1 Customer Validation
Propargyl-PEG4-acid is a PEG-based PROTAC linker can be used in the synthesis of BTK-IAP PROTACs Ibrutinib (HY-10997)-based PROTAC 2 and an analogue PROTAC 3. PROTAC 3 causes BTK degradation with a DC50 of 200 nM in THP-1 cells. Propargyl-PEG4-acid is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 99.70%
- CAS No.: 1415800-32-6
- Formula: C12H20O6
- Molecular Weight:260.28
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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HY-130379
Propargyl-PEG8-acidADC/PROTAC Linker -
HY-130504
Propargyl-PEG1-acidPROTAC Linkers -
HY-126975
Propargyl-PEG3-acidADC/PROTAC Linker -
HY-101157
Propargyl-PEG5-acidADC Linker -
HY-126516
Propargyl-PEG4-NHS esterADC Linker -
HY-118764
Propargyl-PEG2-acidPROTAC/ADC Linker -
HY-101157R
Propargyl-PEG5-acid (Standard)ADC Linker -
HY-140889
Biotin-PEG4-NHS esterPROTAC Linkers -
HY-130386
Propargyl-PEG6-acidADC/PROTAC Linker -
HY-140020
Propargyl-PEG10-acidPEG Derivative -
HY-130383
Propargyl-PEG7-acidADC/PROTAC Linker
All PROTAC Linkers Isoforms
More
Biological Activity
Description
IC50 & Target
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PEGs |
In Vitro
BTK-IAP PROTACs act as stoichiometric degraders, resulting in degradation of BTK protein. Degradation of BTK is a result of IAP E3 ligases family recruitment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1415800-32-6
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Appearance Viscous Liquid
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Molecular Weight 260.28
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Formula C12H20O6
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Color Colorless to light yellow
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SMILES
C#CCOCCOCCOCCOCCC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (192.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8420 mL | 19.2101 mL | 38.4202 mL | 96.0504 mL |
| 5 mM | 0.7684 mL | 3.8420 mL | 7.6840 mL | 19.2101 mL | |
| 10 mM | 0.3842 mL | 1.9210 mL | 3.8420 mL | 9.6050 mL | |
| 15 mM | 0.2561 mL | 1.2807 mL | 2.5613 mL | 6.4034 mL | |
| 20 mM | 0.1921 mL | 0.9605 mL | 1.9210 mL | 4.8025 mL | |
| 25 mM | 0.1537 mL | 0.7684 mL | 1.5368 mL | 3.8420 mL | |
| 30 mM | 0.1281 mL | 0.6403 mL | 1.2807 mL | 3.2017 mL | |
| 40 mM | 0.0961 mL | 0.4803 mL | 0.9605 mL | 2.4013 mL | |
| 50 mM | 0.0768 mL | 0.3842 mL | 0.7684 mL | 1.9210 mL | |
| 60 mM | 0.0640 mL | 0.3202 mL | 0.6403 mL | 1.6008 mL | |
| 80 mM | 0.0480 mL | 0.2401 mL | 0.4803 mL | 1.2006 mL | |
| 100 mM | 0.0384 mL | 0.1921 mL | 0.3842 mL | 0.9605 mL |
Propargyl-PEG4-acid Related Classifications
HY-130481 Related Classifications
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