Expression of two human acyl-CoA:diacylglycerol acyltransferase isozymes in yeast and selectivity of microbial inhibitors toward the isozymes

  • J Antibiot (Tokyo). 2009 Jan;62(1):51-4. doi: 10.1038/ja.2008.5.
Junji Inokoshi  1 ,  Kyosuke Kawamoto ,  Yoichi Takagi ,  Maki Matsuhama ,  Satoshi Omura ,  Hiroshi Tomoda
Affiliations
  • 1. School of Pharmacy, Kitasato University, Tokyo, Japan.
PMID: 19132064 DOI: 10.1038/ja.2008.5
Abstract

Two isozymes for human acyl-coenzyme A:diacylglycerol Acyltransferase (DGAT), DGAT1 and DGAT2, were independently expressed in DGAT-deficient Saccharomyces cerevisiae to establish DGAT1- and DGAT2-S. cerevisiae. The selectivity of DGAT inhibitors of natural origin towards the isozymes was assessed in enzyme assays using the microsomal fractions prepared from DGAT1- and DGAT2-S. cerevisiae. Amidepsines and xanthohumol inhibited DGAT1 and DGAT2 with similar potency, whereas roselipins were found to inhibit DGAT2 selectively.

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