D-sorbose inhibits disaccharidase activity and demonstrates suppressive action on postprandial blood levels of glucose and insulin in the rat

  • Nutr Res. 2014 Nov;34(11):961-7. doi: 10.1016/j.nutres.2014.09.009.
Tsuneyuki Oku  1 ,  Yoko Murata-Takenoshita  2 ,  Yuko Yamazaki  3 ,  Fumio Shimura  3 ,  Sadako Nakamura  4
Affiliations
  • 1. Graduate School of Human Health Science, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan. Electronic address: [email protected].
  • 2. Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan.
  • 3. Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan.
  • 4. Graduate School of Human Health Science, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Faculty of Nursing and Nutrition, Department of Nutrition, University of Nagasaki Siebold, Nagayo, Nagasaki 851-2195, Japan; Institute of Food, Nutrition and Health, Jumonji University, Niiza, Saitama 352-8510, Japan.
PMID: 25311612 DOI: 10.1016/j.nutres.2014.09.009
Abstract

In an attempt to develop D-sorbose as a new sweetener that could help in preventing lifestyle-related diseases, we investigated the inhibitory effect of D-sorbose on disaccharidase activity, using the brush border membrane vesicles of rat small intestines. The inhibitory effect was compared with that of L-sorbose and Other rare sugars, and the small intestinal disaccharidases in rats was compared with that of humans as well. In humans and the small intestines of rats, d-sorbose strongly inhibited sucrase activity and weakly inhibited maltase activity. Inhibition by D-sorbose of sucrase activity was similar to that of L-arabinose, and the K(i) of D-sorbose was 7.5 mM. Inhibition by D-sorbose was very strong in comparison with that of L-sorbose (K(i), 60.8 mM), whereas inhibition of d-tagatose was between that of D-sorbose and L-sorbose. The inhibitory mode of D-sorbose for sucrose and maltase was uncompetitive, and that of L-sorbose was competitive. To determine a suppressive effect on postprandial blood levels of glucose and Insulin via inhibition of sucrase activity, sucrose solution with or without D-sorbose was administered to rats. Increments in the blood levels of glucose and Insulin were suppressed significantly after administration of sucrose solution with D-sorbose to rats, in comparison to administration of sucrose solution without D-sorbose. In contrast, the suppressive effect of L-sorbose on postprandial blood levels of glucose and Insulin was very weak. These results suggest that D-sorbose may have an inhibitory effect on disaccharidase activity and could be used as a sweetener to suppress the postprandial elevation of blood levels of glucose and Insulin. The use of D-sorbose as a sweetener may contribute to the prevention of lifestyle-related diseases, such as type 2 diabetes mellitus.

Keywords
Human; Inhibitory effect; Postprandial blood glucose; Rat disaccharidase; Suppressive effect; d-sorbose.
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