TNF-α-inducing protein of Helicobacter pylori induces epithelial-mesenchymal transition (EMT) in gastric cancer cells through activation of IL-6/STAT3 signaling pathway

  • Biochem Biophys Res Commun. 2017 Mar 4;484(2):311-317. doi: 10.1016/j.bbrc.2017.01.110.
Guodong Chen  1 ,  Na Tang  2 ,  Chao Wang  3 ,  Linqiao Xiao  4 ,  Minjun Yu  5 ,  Lanhua Zhao  6 ,  Hengling Cai  7 ,  Liang Han  8 ,  Chengyuan Xie  9 ,  Yan Zhang  10
Affiliations
  • 1. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 2. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 3. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 4. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 5. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 6. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 7. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 8. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 9. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
  • 10. Institute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, PR China. Electronic address: [email protected].
PMID: 28130110 DOI: 10.1016/j.bbrc.2017.01.110
Abstract

Tumor necrosis factor (TNF)-α-inducing protein (Tipα) is a newly identified carcinogenic factor secreted by Helicobacter pylori (H. pylori). Although it has been proved that Tipα is a strong inducer of epithelial-mesenchymal transition (EMT), a crucial process of migration, the exact molecular mechanism is unknown. Current evidence indicates that the oncogenic transcription factor signal transducers and activators of transcription 3 (STAT3) is inappropriately activated in multiple malignancies, including gastric Cancer. In this study, we showed that Tipα significantly down-regulated the expression of EMT-related markers E-cadherin as well as up-regulated N-Cadherin and vimentin in SGC7901 cells, with typical morphological changes of EMT. Tipα also promoted proliferation and migration of SGC7901 cells. Furthermore, Tipα activated interleukin-6 (IL-6)/STAT3 signaling pathway in SGC7901 cells. The effects of Tipα treatment observed was abolished when we block IL-6/STAT3 signaling pathway. Altogether, our data demonstrated that Tipα may accelerate tumor aggressiveness in gastric Cancer by promoting EMT through activation of IL-6/STAT3 pathway.

Keywords
Epithelial-mesenchymal transition (EMT); Gastric cancer; Helicobacter pylori; IL-6/STAT3 pathway; TNF-α inducing protein (Tipα).
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