Effect of paricalcitol on pancreatic oxidative stress, inflammatory markers, and glycemic status in diabetic rats

  • Ir J Med Sci. 2018 Feb;187(1):75-84. doi: 10.1007/s11845-017-1635-7.
T M Ali  1  2 ,  B El Esawy  3  4 ,  A Elaskary  3  5
Affiliations
  • 1. Department of Clinical Laboratories, College of Applied Medical Sciences, Taif University, P. O. Box 888, Taif, 21974, Saudi Arabia. [email protected].
  • 2. Department of Physiology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt. [email protected].
  • 3. Department of Clinical Laboratories, College of Applied Medical Sciences, Taif University, P. O. Box 888, Taif, 21974, Saudi Arabia.
  • 4. Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
  • 5. Department of Medical Biochemistry, Faculty of Medicine, Al-Azhar University, New Damietta, Egypt.
PMID: 28551720 DOI: 10.1007/s11845-017-1635-7
Abstract

Objectives: This study is designed to explore the effect of paricalcitol (vitamin D receptor agonist) on pancreatic oxidative stress, inflammatory markers, and Adiponectin and glycemic status in diabetic rats.

Materials and methods: Forty Sprague-Dawley male rats aged 10-12 weeks (150-250 g) were used in this study. Type 2 diabetes was developed by providing 4 weeks of high-fat-diet feeding before one shot of streptozotocin injection (40 mg/kg i.p.). Four study groups were designed as normal control rats, diabetic control vehicle-treated, diabetic paricalcitol-treated (0.8 μg/kg), and diabetic glibenclamide-treated (0.6 mg/kg) groups with 10 Animals in each. After treatment of diabetic rats for 3 months, pancreatic inflammatory and oxidative stress markers, plasma Adiponectin, glycemic status parameters, and histopathological pancreatic islet changes were evaluated.

Results: Paricalcitol and glibenclamide treatment significantly (P < 0.05) decreased plasma glucose, Insulin resistance, and pancreatic malondialdehyde and tumor necrosis factor-α levels. Moreover, they significantly (P < 0.05) increased plasma fasting Insulin, C-peptide, Adiponectin, pancreatic IL-2, catalase, superoxide dismutase, Glutathione Peroxidase, and reduced glutathione when contrasted with diabetic control rats. Furthermore, they prevented extensive histopathological damage in the pancreas of diabetic rats.

Conclusions: Paricalcitol reduced pancreatic oxidative stress and inflammatory markers, and improved glycemic status in diabetic rats.

Keywords
Diabetes mellitus; Glibenclamide; Inflammatory markers; Oxidative stress; Pancreas; Paricalcitol; Streptozotocin.
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