Fluorescence assay to predict activity of the glycopeptide antibiotics

  • J Antibiot (Tokyo). 2019 Feb;72(2):114-117. doi: 10.1038/s41429-018-0120-5.
Vladimir Vimberg  1 ,  Radek Gazak  2 ,  Zsolt Szűcs  3 ,  Aniko Borbás  3 ,  Pal Herczegh  3 ,  Jorunn Pauline Cavanagh  4  5 ,  Leona Zieglerova  2 ,  Jan Závora  6 ,  Václava Adámková  6 ,  Gabriela Balikova Novotna  2
Affiliations
  • 1. Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic. [email protected].
  • 2. Institute of Microbiology v. v. i., Czech Academy of Sciences, Vestec, Czech Republic.
  • 3. Department of Pharmaceutical Chemistry, University of Debrecen, Debrecen, Hungary.
  • 4. Department of Pediatrics, University Hospital of North Norway, Tromsø, Norway.
  • 5. Department of Clinical Medicine, UiT The Arctic University of Norway, Tromsø, Norway.
  • 6. Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic.
PMID: 30504918 DOI: 10.1038/s41429-018-0120-5
Abstract

Here, we describe a fluorescent assay developed to study competitive binding of the Glycopeptide antibiotics to live bacteria cells. This assay demonstrated that the mechanism of action of the lipoglycopeptide Antibiotics strongly depends on the hydrophobicity of the substitutes, with the best Antibacterial activity of the Glycopeptide antibiotics equally sharing properties of binding to D-Ala-D-Ala residues of the nascent peptidoglycan and to the membrane.

Products