Effect of Yiqi Huoxue Granules on Platelet Activation Induced by Thrombin

  • Evid Based Complement Alternat Med. 2021 Jul 16:2021:6622848. doi: 10.1155/2021/6622848.
Zhen Lei  1  2 ,  Shuibo Gao  1  2 ,  Xinzhou Wang  1  2 ,  Haixia Gao  1  2 ,  Yongjun Han  1  2 ,  Zhentao Wang  3 ,  Hong Wu  1  2  3
Affiliations
  • 1. Central Laboratory, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450002, China.
  • 2. Laboratory of Cell Imaging, Henan University of Chinese Medicine, Zhengzhou 450002, China.
  • 3. Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou 450002, China.
PMID: 34335832 DOI: 10.1155/2021/6622848
Abstract

Objective: To study the effects of Yiqi Huoxue (YQHX) granules on platelet activation and aggregation induced by Thrombin.

Methods: The effect of YQHX on platelet aggregation rate was detected by platelet aggregation instrument; the effect of YQHX on thrombosis time was observed by the mouse mesentery thrombosis model. DAMI cells were induced to transform into platelet-like granules using PMA, and the effects of SCH (PAR-1 inhibitor) on thrombin-induced changes in platelet intracellular calcium concentration, PAR-1 protein expression, and phosphorylation of MAPK were examined.

Results: Compared with the control group, the platelet aggregation rate, PAR-1 protein expression, phosphorylation of ERK1/2, and p38 protein in the YQHX group decreased (P < 0.05), and there was no significant difference between the YQHX + SCH group and YQHX group (P > 0.05).

Conclusion: YQHX suppresses the platelet activation induced by Thrombin by inhibiting PAR-1 expression.

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