Green Tea Polyphenols Prevent Early Vascular Aging Induced by High-Fat Diet via Promoting Autophagy in Young Adult Rats

  • Curr Med Sci. 2022 Jul 27. doi: 10.1007/s11596-022-2604-6.
Xiang-Tian Xiao  1 ,  Shui-Qing He  2 ,  Nan-Nan Wu  3 ,  Xue-Chun Lin  4 ,  Jing Zhao  4 ,  Chong Tian  5
Affiliations
  • 1. Medical College of Wuhan University of Science and Technology, Wuhan, 430081, China.
  • 2. Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. [email protected].
  • 3. School of Public Health and Management, Research Center for Medicine and Social Development, Innovation Center for Social Risk Governance in Health, Chongqing Medical University, Chongqing, 400016, China.
  • 4. Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • 5. School of Nursing, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
PMID: 35896932 DOI: 10.1007/s11596-022-2604-6
Abstract

Objective: Epidemiology studies indicate that green tea Polyphenols (GTP) perform a protective effect on cardiovascular diseases, but the underlying mechanisms are complex. The present study aimed to investigate the effect of GTP on high-fat diets (HFD) induced-early vascular aging.

Methods: Six-week young adult Wistar rats were fed with standard chow or HFD in the presence and absence of GTP (200 mg/kg body weight) for 18 weeks. In vitro experiment, human umbilical vascular endothelial cells (HUVECs) were treated with palmitic acid (PA) and GTP.

Results: The results showed that GTP alleviated the disorganized arterial wall and the increased intima-media thickness induced by HFD. In addition, the vascular oxidative injury was suppressed following GTP treatment. Furthermore, GTP elevated the ratio of LC3-II/LC3-I and suppressed expression of p62/SQSTM1, and restored SIRT3 expression in the aorta of HFD rats. Consistently, in cultured HUVECs, GTP inhibited cell senescence indicated by SA-β-gal and promoted endothelial Autophagy compared with the PA treatment group. The activity of SIRT3 was specifically inhibited by 3-TYP, and the protective effect of GTP was consequently abolished.

Conclusion: The findings indicated that GTP protected against early vascular senescence in young HFD rats via ameliorating oxidative injury and promoting Autophagy which was partially regulated by the SIRT3 pathway.

Keywords
Sirtuin 3; autophagy; early vascular aging; green tea polyphenols; high-fat diets.
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