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Cat. No.: HY-139012

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RC574

RC574 is a GPx1 and GPx4 modulator that increases GPx1 protein levels and enzyme activity and protects cells against RSL3-induced GPx4 inactivation and ferroptosis. RC574 inhibits glutamate-induced ferroptosis/oxytosis, mitochondrial superoxide anion production, mitochondrial membrane hyperpolarization, oxidant production, and mitochondrial fragmentation. RC574 protects primary cortical neurons against glutamate challenge and inhibits mitochondrial superoxide anion production while increasing antioxidant enzyme activity. RC574 ameliorates motor-related behavioral deficits and reduces reactive astrocytes in the striatum in a rat model of ischemic stroke. RC574 can be used in research on neurodegenerative diseases and ischemic stroke.

For research use only. We do not sell to patients.

CAS No.: 2584411-87-8
Formula: C₁₈H₂₄OSSe
Molecular Weight:367.41

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

RC574 Chemical Structure

CAS No.: 2584411-87-8

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

RC574 is a GPx1 and GPx4 modulator that increases GPx1 protein levels and enzyme activity and protects cells against RSL3-induced GPx4 inactivation and ferroptosis. RC574 inhibits glutamate-induced ferroptosis/oxytosis, mitochondrial superoxide anion production, mitochondrial membrane hyperpolarization, oxidant production, and mitochondrial fragmentation. RC574 protects primary cortical neurons against glutamate challenge and inhibits mitochondrial superoxide anion production while increasing antioxidant enzyme activity. RC574 ameliorates motor-related behavioral deficits and reduces reactive astrocytes in the striatum in a rat model of ischemic stroke. RC574 can be used in research on neurodegenerative diseases and ischemic stroke^[1]^[2].

IC₅₀ & Target

^[1]

GPX1

GPX4

All Glutathione Peroxidase Isoforms :

GPX1
GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
HT-22	IC50	276.2 nM	Protection against 10 mM glutamate-induced oxidative toxicity in mouse hippocampal HT22 cells assessed as cell viability using CellTiter-Blue (CTB) reagent incubated for 24 hrs. Protection against 10 mM glutamate-induced oxidative toxicity in mouse hippocampal HT22 cells assessed as cell viability using CellTiter-Blue (CTB) reagent incubated for 24 hrs.	32514861

In Vitro

Glutamate Release or ReceptorsOxidative StressMitochondrial FunctionFerroptosis

Glutamate Release or Receptors

RC574 (0.0625-3 μM; 24 h) exerts a partial protective effect against glutamate-induced oxidative toxicity in HT22 cells, with an IC₅₀ of 276.2 nM^[1].
RC574 (3 μM; 24 h) protects primary mouse cortical neurons against glutamate-induced toxicity^[1].

Glutamate Release or Receptors

Oxidative Stress

RC574 (3 μM; 24 h) partially reduced glutamate-induced oxidant production in HT22 cells^[1].

Mitochondrial Function

Glutamate Release or Receptors

RC574 (3 μM; 24 h) prevents glutamate-induced mitochondrial membrane hyperpolarization in HT22 cells^[1].
RC574 (3 μM; 24 h) reduces the basal level of fragmented mitochondria in glutamate-challenged HT22 cells and maintains mitochondrial morphology^[1].

Mitochondrial Function

Oxidative Stress

Glutamate Release or Receptors

RC574 (3 μM; 24 h) inhibits glutamate-induced mitochondrial superoxide anion production in HT22 cells^[1].

Oxidative Stress

Glutamate Release or Receptors

RC574 (3 μM; 30 h) increases GPx1 protein levels and GPx activity in HT22 cells^[1].

Ferroptosis

Glutamate Release or Receptors

RC574 (0.0625-5 μM; 24 h) protects HT22 cells from RSL3 (HY-100218A)-induced ferroptosis, with complete protection at 62.5 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

HT22
Concentration:

0.0625, 0.125, 0.25, 0.5, 1 μM
Incubation Time:

24 h
Result:

Partially protected against 10 mM glutamate-induced toxicity.
Was fully protective at 0.5 μM with a calculated IC₅₀ of 276.2 nM.

In Vivo

RC574 effectively improves motor-related behavioral deficits and reduces reactive astrocytes in the striatum in a rat model of ischemic stroke^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 2584411-87-8
Molecular Weight 367.41
Formula C₁₈H₂₄OSSe
SMILES

OC1=C(C(C)(C)C)C=C([Se]C2=CC=CS2)C=C1C(C)(C)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bueno DC, et al. New Probucol Analogues Inhibit Ferroptosis, Improve Mitochondrial Parameters, and Induce Glutathione Peroxidase in HT22 Cells. Molecular neurobiology. 2020 Aug;57(8):3273-3290. [Content Brief]

[2]. Sharif A, et al. The therapeutic potential of probucol and probucol analogues in neurodegenerative diseases. Translational neurodegeneration. 2024 Jan 22;13(1):6.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References