CTLA-4 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The CTLA-4 protein is a key inhibitory receptor and a major negative regulator of T cell responses in coordination of immune regulation. Its unique property is its significantly increased affinity for B7 ligands (CD80/CD86) compared with CD28. CTLA-4 Protein, Human (HEK293, His) is the recombinant human-derived CTLA-4 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CTLA-4 protein is a key inhibitory receptor and a major negative regulator of T cell responses in coordination of immune regulation. Its unique property is its significantly increased affinity for B7 ligands (CD80/CD86) compared with CD28. CTLA-4 Protein, Human (HEK293, His) is the recombinant human-derived CTLA-4 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
GMP CTLA-4, a pivotal inhibitory receptor, emerges as a principal negative regulator orchestrating T-cell responses within the intricate framework of immune modulation. This regulatory function stems from the distinctive property of GMP CTLA-4, displaying significantly heightened affinity for its natural B7 family ligands, CD80 and CD86, compared to the cognate stimulatory coreceptor CD28. This pronounced difference in binding affinity positions GMP CTLA-4 to competitively engage with CD80/B7-1 and CD86/B7.2, exerting a suppressive influence on T-cell activation and finely tuning immune responses. The homodimeric structure of GMP CTLA-4, intricately linked by disulfide bonds, further underscores its role as a molecular sentinel in immune regulation. Additionally, GMP CTLA-4 interacts with ICOSLG, contributing to its multifaceted engagement in immune checkpoint pathways.
Verified Bioactivity
1.Immobilized Mouse B7-1-Fc at 10 μg/mL (100 μL/well) can bind Human CTLA-4-His with an ED50 value of 5-50 ng/mL.
2. Loaded Botensilimab (HY-P99484) on AHC2 biosensor, can bind CTLA-4 Protein, Human (HEK293, His, HY-P70684) with an affinity constant of 8.815E-10 M as determined in BLI assay.
3. Immobilized Mouse B7-2-Fc at 2 μg/mL (100 μL/well) can bind Human CTLA-4-His. The ED50 of Recombinant Human CTLA-4-His is 5-50 ng/mL.
MCE Validation Data
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Bioactivity - BLI
Bioactivity - BLI
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P16410 (K36-D161)
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Molecular Construction
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N-term
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CTLA-4 (K36-D161)
Accession # P16410 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CTLA4; Celiac Disease 3; Prev. CELIAC3; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Prev. IDDM12; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; CTLA-4; Cytotoxic T-Lymphocyte-Associated Protein 4; CD152; Cytotoxic T-Lymphocyte A
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AA Sequence
KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD
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Predicted Molecular Mass
14.3 kDa
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Molecular Weight
Approximately 20-25 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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