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Cat. No.: HY-W127781

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Rhod-2 triammonium

Rhod-2 triammonium is a cell impermeant, red fluorescent calcium indicator. Rhod-2 triammonium exhibits a significant shift in fluorescence intensity upon calcium binding (ex max=549 nm; calcium-free v. ex/em max=552/581 nm; calcium-bound). Unlike the UV-excitable indicators Fura-2 and Indo-1 (HY-D0121), there is no accompanying spectral shift.

For research use only. We do not sell to patients.

CAS No.: 129787-65-1
Formula: C₃₉H₄₉N₇O₁₁
Molecular Weight:791.85

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

Rhod-2 triammonium Chemical Structure

CAS No.: 129787-65-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Because Rhod-2 triammonium cannot permeate the cell membrane, it can be permeabilized with 0.01% saponin or microinjected before cell incubation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 129787-65-1
Molecular Weight 791.85
Formula C₃₉H₄₉N₇O₁₁
SMILES

O=C(CN(CC(O)=O)C1=C(OCCOC2=C(N(CC(O)=O)CC(O)=O)C=CC=C2)C=C(C3=C4C=CC(N(C)C)=CC4=[O+]C5=C3C=CC(N(C)C)=C5)C=C1)[O-].N.N.N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Minta, et al. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J. Biol. Chem. 264(14), 8171-8178 (1989).

[2]. Maxwell JT, et al. A novel mechanism of tandem activation of ryanodine receptors by cytosolic and SR luminal Ca2+ during excitation-contraction coupling in atrial myocytes. J Physiol. 2017 Jun 15;595(12):3835-3845. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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