RJW103 TFA
RJW103 TFA is an acid-stable SF-1 (NR5A1) and LRH-1 agonist with pEC50 values of 6.5 and 5.9, respectively. RJW103 TFA activates SF-1- and LRH-1-mediated transcription of endogenous target genes. RJW103 TFA can be used in research on cancer, endocrinology and metabolic diseases, such as adrenocortical tumors.
For research use only. We do not sell to patients.
- Formula: C30H36F3NO2
- Molecular Weight:499.61
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
RJW103 TFA (10 μM; 24 h) activates SF-1-mediated transcription of SHP, StAR, G0S2, and MeOX1 in mSF-1-expressing HEK293 cells, and activates LRH-1-mediated transcription of StAR and G0S2 (but not SHP or MeOX1) in hLRH-1-expressing HEK293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293 cells stably expressing mouse SF-1, HEK293 cells stably expressing human LRH-1
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Concentration:10 μM
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Incubation Time:24 h
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Result:Significantly increased mRNA levels of SHP, StAR, G0S2, and MeOX1 in mouse SF-1-expressing HEK293 cells.
Significantly increased mRNA levels of StAR and G0S2, but did not activate SHP or MeOX1, in human LRH-1-expressing HEK293 cells.
Chemical Information
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Appearance Oil
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Molecular Weight 499.61
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Formula C30H36F3NO2
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SMILES
C=C(C1=CC=CC=C1)[C@]23C(C4=CC=CC=C4)=C(C[C@]2(CN(C3)C)[H])CCCCCC.O=C(O)C(F)(F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (100.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Whitby RJ, et al. Small molecule agonists of the orphan nuclear receptors steroidogenic factor-1 (SF-1, NR5A1) and liver receptor homologue-1 (LRH-1, NR5A2). J Med Chem. 2011;54(7):2266-2281. [Content Brief]
[2]. Wu T, et al. Liver receptor homolog-1: structures, related diseases, and drug discovery. Acta Pharmacol Sin. 2024;45(8):1571-1581. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0016 mL | 10.0078 mL | 20.0156 mL | 50.0390 mL |
| 5 mM | 0.4003 mL | 2.0016 mL | 4.0031 mL | 10.0078 mL | |
| 10 mM | 0.2002 mL | 1.0008 mL | 2.0016 mL | 5.0039 mL | |
| 15 mM | 0.1334 mL | 0.6672 mL | 1.3344 mL | 3.3359 mL | |
| 20 mM | 0.1001 mL | 0.5004 mL | 1.0008 mL | 2.5020 mL | |
| 25 mM | 0.0801 mL | 0.4003 mL | 0.8006 mL | 2.0016 mL | |
| 30 mM | 0.0667 mL | 0.3336 mL | 0.6672 mL | 1.6680 mL | |
| 40 mM | 0.0500 mL | 0.2502 mL | 0.5004 mL | 1.2510 mL | |
| 50 mM | 0.0400 mL | 0.2002 mL | 0.4003 mL | 1.0008 mL | |
| 60 mM | 0.0334 mL | 0.1668 mL | 0.3336 mL | 0.8340 mL | |
| 80 mM | 0.0250 mL | 0.1251 mL | 0.2502 mL | 0.6255 mL | |
| 100 mM | 0.0200 mL | 0.1001 mL | 0.2002 mL | 0.5004 mL |
RJW103 TFA Related Classifications
HY-134462A Related Classifications
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