2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Apoptosis
  3. Ser/Thr Protease Hepcidin Reactive Oxygen Species (ROS) Apoptosis
  4. RLYB331

RLYB331 (KY1066) is a is a monoclonal antibody targeting Matriptase-2 (TMPRSS6). RLYB331 binds to the serine protease active site of matriptase-2, blocking protease activity to increase hepcidin expression. RLYB331 reduces iron overload, inhibits α-aggregates, reduces ROS, inhibits apoptosis, and enhances erythroid differentiation. RLYB331 ameliorates ineffective erythropoiesis in β-thalassemic mouse models. RLYB331 can be used for the research of β-thalassemia.

For research use only. We do not sell to patients.

RLYB331

RLYB331 Chemical Structure

Size Stock
1 mg   Get quote  
5 mg   Get quote  
10 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

RLYB331 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

RLYB331 (KY1066) is a is a monoclonal antibody targeting Matriptase-2 (TMPRSS6). RLYB331 binds to the serine protease active site of matriptase-2, blocking protease activity to increase hepcidin expression. RLYB331 reduces iron overload, inhibits α-aggregates, reduces ROS, inhibits apoptosis, and enhances erythroid differentiation. RLYB331 ameliorates ineffective erythropoiesis in β-thalassemic mouse models. RLYB331 can be used for the research of β-thalassemia[1][2].

Species Reactivity

Human
In Vivo

RLYB331 (10 mg/kg; i.p.; once weekly; 8 weeks) increases hepcidin expression, reduces iron overload, eliminates α-globin aggregates, improves erythroid differentiation, reduces splenomegaly, and corrects reticulocytosis in Hbbth3/+ β-thalassemic mice[1].
RLYB331 (10 mg/kg; i.p.; single dose) increases hepcidin expression via enhanced Bmp/Smad signaling in healthy C57BL/6 mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: β-thalassemic (Hbbth3/+ ) mice and wild-type C57BL/6N mice (8-week-old; male)[1]
Dosage: 10 mg/kg
Administration: i.p.; once weekly; 8 weeks
Result: Increased liver Hamp mRNA by 2.24-fold.
Increased serum hepcidin levels compared to isotype control.
Reduced serum iron levels and restored liver iron content to normal WT ranges.
Decreased liver iron staining by Perl's Prussian blue.
Reduced the number of erythroid cells in spleen fractions III (polychromatic) and IV (orthochromatic cells/reticulocytes).
Increased the number of terminally differentiated RBCs (fraction V) in spleen.
Reduced reactive oxygen species (ROS) to near-normal levels in spleen.
Reduced the percentage of apoptotic (annexin V+) erythroid cells in spleen.
Increased the number of terminally differentiated RBCs (fraction V) in bone marrow.
Eliminated detectable membrane-associated α-globin aggregates in circulating RBCs.
Corrected reticulocytosis.
Significantly reduced splenomegaly.
Did not significantly increase hemoglobin levels, hematocrit, or RBC counts compared to isotype control.
Animal Model: C57BL/6[1]
Dosage: 10 mg/kg
Administration: i.p.; single dose
Result: Increased Bmp/Smad signaling.
Increased hepcidin expression.
Gene ID

164656  [NCBI]

Accession
Target

TMPRSS6
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

RLYB331 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RLYB331KY1066RLYB 331RLYB-331KY 1066KY-1066Ser/Thr ProteaseHepcidinReactive Oxygen Species (ROS)ApoptosisSerine proteasesSerine endopeptidasesThreonine proteasesα-aggregateshepcidinserine protease active siteerythroid differentiationmatriptase-2iron overloadTMPRSS6β-thalassemiaapoptosisROSInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
RLYB331
Cat. No.:
HY-P992451
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.