2. Cell Cycle/DNA Damage
  3. Cyclin G-associated Kinase (GAK)
  4. Romiplostim

Romiplostim is an Fc-peptide fusion protein mimetic of TPO (thrombopoietin protein) and also a c-Mpl agonist. Romiplostim binds to c-Mpl, stimulates megakaryocyte-mediated thrombopoiesis, and activates the same downstream signaling pathways as endogenous TPO. Romiplostim is applicable to research related to hematopoietic acute radiation syndrome and chronic immune thrombocytopenia.

For research use only. We do not sell to patients.

CAS No. : 267639-76-9

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Description

Romiplostim is an Fc-peptide fusion protein mimetic of TPO (thrombopoietin protein) and also a c-Mpl agonist. Romiplostim binds to c-Mpl, stimulates megakaryocyte-mediated thrombopoiesis, and activates the same downstream signaling pathways as endogenous TPO. Romiplostim is applicable to research related to hematopoietic acute radiation syndrome and chronic immune thrombocytopenia[1].

IC50 & Target

TPO (thrombopoietin protein)
Parmacokinetics
Species Dose Route Tmax Cmax T1/2 AUC0-t
Mice[1] 30 μg/kg s.c. 12 h 6 ng/mL 24.3 h 228 ng·h/mL
Mice[1] 300 μg/kg s.c. 4 h 201 ng/mL 29.9 h 8912 ng·h/mL
In Vivo

Romiplostim (3-300 μg/kg; s.c.; single dose at 24-26 h post-irradiation; daily on Days 1-3 post-irradiation; daily on Days 1-5 post-irradiation) provides a statistically significant ~40% increase in 30-day survival and hastens platelet recovery when administered as a single subcutaneous dose of 30 or 100 μg/kg 24 hours post-6.8 Gy total body irradiation, with no additional benefit from repeat dosing or co-administration with pegfilgrastim[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6J (male and female, 11-12 weeks of age, total body irradiation with 6.8 Gy X-rays)[1]
Dosage: 3 μg/kg; 10 μg/kg; 30 μg/kg; 100 μg/kg; 300 μg/kg
Administration: s.c.; single dose at 24-26 h post-irradiation; daily on Days 1-3 post-irradiation; daily on Days 1-5 post-irradiation
Result: Resulted in 41% 30-day survival (3 μg/kg single dose, 6.8 Gy model).
Resulted in 38% 30-day survival (10 μg/kg single dose, 6.8 Gy model).
Resulted in 57% 30-day survival (30 μg/kg single dose, 6.8 Gy model).
Resulted in 52% 30-day survival (100 μg/kg single dose, 6.8 Gy model).
Resulted in 71% 30-day survival (30 μg/kg single dose, 6.8 Gy non-bled cohort).
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

59 kDa

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Fusion - IGHG1 Fc (Fragment constant) - [peptide 14-mer - peptide 14-mer]2
Biological Activity
  • Loaded Romiplostim on AHC2 biosensor, can bind C-MPL Protein, Human (HEK293, His, HY-P78002) with an affinity constant of 3.144E-08 M as determined in BLI assay.
Purity & Documentation

Purity: 99%

SDS (251 KB)

COA (232 KB) SDS-PAGE (113 KB) SEC-HPLC (520 KB)

Inhibitory Antibodies User Guide (603 KB)
References
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Romiplostim Related Classifications

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  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Romiplostim267639-76-9Cyclin G-associated Kinase (GAK)Auxilin 2hematopoietic acute radiation syndrometotal body irradiationchronic immune thrombocytopeniaplateletsmegakaryocyteTPO receptor (c-Mpl)pegfilgrastimirradiated miceInhibitorinhibitorinhibit

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