S-(5'-Adenosyl)-L-methionine chloride
S-(5'-Adenosyl)-L-methionine chloride is the endogenous methyl donor in the methionine cycle and numerous methylation reactions, including protein methylation. S-(5'-Adenosyl)-L-methionine chloride can activate cardiac ryanodine receptor RyR2 in a concentration-dependent manner, enhancing the affinity of RyR2 for ryanodine. The Kd of S-(5'-Adenosyl)-L-methionine chloride with MetJ in Escherichia coli is 0.18 mM. S-(5'-Adenosyl)-L-methionine chloride can cause changes in the methylation index of Cisplatin (HY-17394)-sensitive and Cisplatin-resistant ovarian cancer cells. S-(5'-Adenosyl)-L-methionine chloride can be used for methylation-related research.
For research use only. We do not sell to patients.
- CAS No.: 24346-00-7
- Formula: C15H23ClN6O5S
- Molecular Weight:434.90
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
S-(5'-Adenosyl)-L-methionine chloride (SAM) has a dissociation constant Kd=0.18 mM among the GST-MetJ ligands determined by FAC-MS, and its binding affinity for MetJ is higher than that of adenosine-type metabolites such as Ade, MTA, SAC, SAH, and Ado[1].
S-(5'-Adenosyl)-L-methionine chloride (SAM) changed the SAM/SAH methylation index from 2.5 to 4.5 in A2780 cytosolic extracts and from 10.3 to 4.0 in A2780CIS cytosolic extracts, reflecting changes in SAM levels in these ovarian cancer cell lines[2].
S-(5'-Adenosyl)-L-methionine chloride activates cardiac RyR2 in porcine CSR vesicles by increasing ryanodine binding affinity, with significant activation at ≥75 µM and reducing the ryanodine Kd to 9.5 at 0.1 mM SAM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 24346-00-7
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Molecular Weight 434.90
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Formula C15H23ClN6O5S
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SMILES
O[C@@H]1[C@H](O)[C@@H](C[S+](C)CC[C@H](N)C([O-])=O)O[C@H]1N2C=NC3=C(N=CN=C32)N.Cl
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Structure Classification
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Initial Source
Saccharomyces cerevisiae
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Calcium Spark Assay
Calcium sparks are localized, transient increases in intracellular calcium concentration ([Ca2+]i) that occur in cardiac myocytes and represent elementary events underlying excitation-contraction coupling. These events are generated by the coordinated opening of clusters of ryanodine receptors (RyRs) on the sarcoplasmic reticulum membrane, leading to a brief release of Ca2+ into the cytosol. The detection and analysis of calcium sparks provide insights into the mechanisms of calcium handling and signaling in cardiac cells. Imaging techniques using fluorescent calcium indicators such as Fluo-3 are employed to visualize these subcellular calcium transients with high spatial and temporal resolution. The protocol is based on established methodologies described in primary literature for both experimental measurement and automated analysis of calcium sparks.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
S-(5'-Adenosyl)-L-methionine chloride Related Classifications
HY-W338810 Related Classifications
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