2. NF-κB Neuronal Signaling
  3. NF-κB Beta-secretase
  4. (S)-(-)-Anatabine

(S)-(-)-Anatabine is an NFκB/BACE-1 inhibitor with blood-brain barrier penetration. (S)-(-)-Anatabine inhibits NFκB activation via phosphorylation of its p65 subunit. (S)-(-)-Anatabine inhibits BACE-1 transcription and reduces BACE-1 protein levels. (S)-(-)-Anatabine lowers production of Aβ1-40 and Aβ1-42 by reducing β-cleavage of amyloid precursor protein without affecting α-cleavage. (S)-(-)-Anatabine can be used for the research of Alzheimer's disease.

For research use only. We do not sell to patients.

(S)-(-)-Anatabine

(S)-(-)-Anatabine Chemical Structure

CAS No. : 581-49-7

Size Price Stock
5 mg Ask For Quote & Lead Time
10 mg Ask For Quote & Lead Time
25 mg Ask For Quote & Lead Time
50 mg Ask For Quote & Lead Time
100 mg Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of (S)-(-)-Anatabine:

Other Forms of (S)-(-)-Anatabine:

(S)-(-)-Anatabine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (S)-(-)-Anatabine

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Beta-secretase Isoform Specific Products:

View All Isoforms
BACE1 BACE2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(S)-(-)-Anatabine is an NFκB/BACE-1 inhibitor with blood-brain barrier penetration. (S)-(-)-Anatabine inhibits NFκB activation via phosphorylation of its p65 subunit. (S)-(-)-Anatabine inhibits BACE-1 transcription and reduces BACE-1 protein levels. (S)-(-)-Anatabine lowers production of Aβ1-40 and Aβ1-42 by reducing β-cleavage of amyloid precursor protein without affecting α-cleavage. (S)-(-)-Anatabine can be used for the research of Alzheimer's disease[1].

IC50 & Target[1]

NF-κB

 

BACE1

 

In Vitro

(S)-(-)-Anatabine (24 h) dose-dependently reduces Aβ1-40 and Aβ1-42 production with an IC50 of 640 μg/mL[1].
(S)-(-)-Anatabine (200-800 μg/mL; 3 h) dose-dependently inhibits TNFα-induced NFκB activation in HEK293 NFκB luciferase reporter cells[1].
(S)-(-)-Anatabine (600 μg/mL; 24 h) inhibits basal p65 NFκB phosphorylation in 7W CHO cells overexpressing human APP, and inhibits TNFα-induced p65 NFκB phosphorylation in HEK293 and SHSY-5Y cells[1].
(S)-(-)-Anatabine (500-1000 μg/mL; 30 min) fully inhibits TNFα-induced BACE-1 transcription in human neuronal-like SHSY-5Y cells[1].
(S)-(-)-Anatabine (5-1000 μg/mL; 24 h) dose-dependently reduces BACE-1 protein levels in human neuronal-like SHSY-5Y cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(S)-(-)-Anatabine Related Antibodies

Western Blot Analysis[1]

Cell Line: 7W CHO cells overexpressing human APP, HEK293 cells, human neuronal-like SHSY-5Y cells
Concentration: 600 μg/mL
Incubation Time: 24 h
Result: Significantly reduced basal p65 NFκB phosphorylation relative to controls in 7W CHO cells.
Significantly reduced TNFα-induced p65 NFκB phosphorylation relative to TNFα-only treatment in HEK293 and SHSY-5Y cells.

Real Time qPCR[1]

Cell Line: human neuronal-like SHSY-5Y cells
Concentration: 500 μg/mL; 1000 μg/mL
Incubation Time: 30 min
Result: Fully prevented the TNFα-induced increase in BACE-1 mRNA levels at both 500 μg/mL and 1000 μg/mL.
Showed no significant difference in BACE-1 mRNA levels between control cells and cells treated with TNFα + anatabine.

Western Blot Analysis[1]

Cell Line: human neuronal-like SHSY-5Y cells
Concentration: 5 μg/mL; 20 μg/mL; 50 μg/mL; 100 μg/mL; 200 μg/mL; 800 μg/mL; 1000 μg/mL
Incubation Time: 24 h
Result: Reduced BACE-1 protein levels dose-dependently.
Caused statistically significant reductions in BACE-1 protein levels relative to controls at all doses higher than 50 μg/mL.
Parmacokinetics
Species Dose Route AUC0-∞ (Brain) Cmax (Brain) AUC0-∞ (Plasma) Cmax
Mice[1] 2 mg/kg i.v. 172384 min·ng/mL 5194.4 ng/mL 49968 min·ng/mL 1501.7 ng/mL
In Vivo

(S)-(-)-Anatabine (2 mg/kg; i.p.; single dose) can cross the blood–brain barrier, with the area under the curve (AUC)
and the maximal concentration (Cmax) achieving approximately 3.5 times greater in the brain of B6/SJL F1 mice than in the plasma[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: B6/SJL F1 mice (43 week-old)[1]
Dosage: 2 mg/kg
Administration: i.p.; single dose
Result: Exhibited AUC of 172384.9 ng·min/mL and 49968 ng·min/mL in the brain and plasma, respectively.
Exhibited Cmax of 5194.4 ng/mL and 1501.7 ng/mL in the brain and plasma, respectively.
Molecular Weight

160.22

Formula

C10H12N2
CAS No.
SMILES

[C@@H]1(C2=CC=CN=C2)CC=CCN1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

(S)-(-)-Anatabine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(S)-(-)-Anatabine581-49-7NF-κBBeta-secretaseNuclear factor-κBNuclear factor-kappaBBACEβ-SecretaseBACE-1SHSY-5Y cellsNFκBtransgenic Alzheimer's disease mouse modelamyloid precursor proteinblood-brain barrier7W CHO cellsp65 subunitAβ1-40Aβ1-42Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
(S)-(-)-Anatabine
Cat. No.:
HY-126047
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.