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  2. Safranin

Safranin (Synonyms: Safranin O; Basic Red 2)

Cat. No.: HY-D0215
Handling Instructions

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration.

For research use only. We do not sell to patients.

Safranin Chemical Structure

Safranin Chemical Structure

CAS No. : 477-73-6

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1 g USD 50 In-stock
Estimated Time of Arrival: December 31
5 g USD 150 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration[1].

Molecular Weight

350.84

Formula

C20H19ClN4

CAS No.
SMILES

CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (71.26 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8503 mL 14.2515 mL 28.5030 mL
5 mM 0.5701 mL 2.8503 mL 5.7006 mL
10 mM 0.2850 mL 1.4252 mL 2.8503 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (7.13 mM); Clear solution; Need ultrasonic

*All of the co-solvents are available by MCE.
References
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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

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Product Name:
Safranin
Cat. No.:
HY-D0215
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