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  4. Safranin

Safranin  (Synonyms: Safranine T)

Cat. No.: HY-D0215
COA Handling Instructions

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration.

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Safranin Chemical Structure

Safranin Chemical Structure

CAS No. : 477-73-6

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Based on 1 publication(s) in Google Scholar

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Description

Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. It can readily intercalate into biological macromolecules, including DNA and proteins. Safranin can be used as a redox indicator in the determination of metal ion concentration[1].

Molecular Weight

350.84

Appearance

Solid

Formula

C20H19ClN4
CAS No.
SMILES

CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (71.26 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8503 mL 14.2515 mL 28.5030 mL
5 mM 0.5701 mL 2.8503 mL 5.7006 mL
10 mM 0.2850 mL 1.4252 mL 2.8503 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (7.13 mM); Clear solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References
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Safranin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Safranin477-73-6Safranine TFluorescent Dyephenazinium dye spectroscopic proberedox indicatorInhibitorinhibitorinhibit

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