Salvicine
Salvicine is a DNA topoisomerase II inhibitor (IC50=3 μM). Salvicine increases the interaction between DNA and Topo II by interacting with the ATPase domain, inhibiting DNA degradation and ATP hydrolysis. Salvicine has anticancer activities, including inhibiting Topo II, causing DNA damage, overcoming multidrug resistance, and inhibiting tumor cell adhesion.
For research use only. We do not sell to patients.
- CAS No.: 240423-23-8
- Formula: C20H26O4
- Molecular Weight:330.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Topoisomerase Isoforms
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Biological Activity
Description
IC50 & Target
[2]|
Topoisomerase II 3 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
3.57 μM
Compound: 4
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In vitro cytotoxic activity against HL-60 human leukemia cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against HL-60 human leukemia cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| P388 | IC50 |
3.49 μM
Compound: 4
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In vitro cytotoxic activity against P388 mouse leukemia cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against P388 mouse leukemia cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| SGC-7901 | IC50 |
1.84 μM
Compound: 4
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In vitro cytotoxic activity against SGC-7901 stomach cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against SGC-7901 stomach cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| SPC-A4 | IC50 |
2.46 μM
Compound: 4
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In vitro cytotoxic activity against SPC-A4 lung cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against SPC-A4 lung cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
Chemical Information
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CAS No. 240423-23-8
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Molecular Weight 330.42
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Formula C20H26O4
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SMILES
O=C1C(C(C(C)C)=CC2=C1C(CCC(C(C)(C)O)O)=C(C)C=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Genotoxicity/Mutagenicity Study
The bacterial reverse mutation assay detects point mutations that restore amino-acid prototrophy in auxotrophic Salmonella typhimurium or Escherichia coli tester strains; after exposure to a test article, mutagenic activity is read out as an increased number of revertant colonies on minimal agar compared with the vehicle control. The assay uses tester strains with different mutation targets so that base-substitution and frameshift mutagens can be detected, and testing is performed with and without exogenous mammalian metabolic activation because some chemicals require biotransformation to become mutagenic.
Purity & Documentation
References
[1]. Dey D, et al. Investigating the anticancer potential of salvicine as a modulator of topoisomerase II and ROS signaling cascade[J]. Frontiers in oncology, 2022, 12: 899009. [Content Brief]
[2]. Meng L, et al. Salvicine, a novel DNA topoisomerase II inhibitor, exerting its effects by trapping enzyme-DNA cleavage complexes[J]. Biochemical pharmacology, 2001, 62(6): 733-741. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Salvicine Related Classifications
HY-W715630 Related Classifications
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