2. Cell Cycle/DNA Damage
  3. SRPK
  4. SPHINX

SPHINX is a selective SRPK1 inhibitor with an IC50 value of 0.58 μM. SPHINX effectively reduces Choroidal Neovascularization (CNV) in vivo. SPHINX can be used for the research of (age-related macular degenaration) AMD.

For research use only. We do not sell to patients.

SPHINX Chemical Structure

SPHINX Chemical Structure

CAS No. : 848057-98-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 308 In-stock
Solution
10 mM * 1 mL in DMSO USD 308 In-stock
Solid
5 mg USD 280 In-stock
10 mg USD 450 In-stock
25 mg USD 850 In-stock
50 mg USD 1350 In-stock
100 mg USD 1950 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

SPHINX Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

SPHINX is a selective SRPK1 inhibitor with an IC50 value of 0.58 μM. SPHINX effectively reduces Choroidal Neovascularization (CNV) in vivo. SPHINX can be used for the research of (age-related macular degenaration) AMD[1].

IC50 & Target

IC50: 0.58 μM (SRPK1)[1]
In Vitro

SPHINX (10 μM; 2 h) affects EGF-induced phosphorylation of SRSF1 and SRSF2[1].
SPHINX (5 μM; 24 h) reduces the expression of VEGF165 relative to GAPDH control either in primary RPE and ARPE-19 cell lines[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SPHINX Related Antibodies

Western Blot Analysis[1]

Cell Line: ARPE-19 cell line
Concentration: 10 μM
Incubation Time: 2 hours
Result: Blocked EGF-induced phosphorylation of SRSF1 and SRSF2.
In Vivo

SPHINX (10 ng; i.o. on laser photocoagulation day 0 and day 7) affects neovascular growth in vivo[1].
SPHINX (25 ng; i.o. on laser photocoagulation day 0 and day 7) affects the CNV area in CNV rats[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57/B6 mice with laser-induced CNV[1]
Dosage: 10 ng
Administration: Intraocular injection; 10 ng on laser photocoagulation day 0 and day 7
Result: Significantly reduced neovascular growth compared with saline-injected controls.
Animal Model: Norway Brown rats with laser-induced choroidal neovascularization[1]
Dosage: 25 ng (10 ng/uL)
Administration: Intraocular injection; 25 ng (10 ng/uL) on laser photocoagulation day 0 and day 7
Result: Significantly reduced the CNV area compared with saline injected controls.
Clinical Trial
Molecular Weight

354.32

Formula

C17H17F3N2O3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C(C)O1)NC2=CC(C(F)(F)F)=CC=C2N3CCOCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (141.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8223 mL 14.1115 mL 28.2231 mL
5 mM 0.5645 mL 2.8223 mL 5.6446 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8223 mL 14.1115 mL 28.2231 mL 70.5577 mL
5 mM 0.5645 mL 2.8223 mL 5.6446 mL 14.1115 mL
10 mM 0.2822 mL 1.4112 mL 2.8223 mL 7.0558 mL
15 mM 0.1882 mL 0.9408 mL 1.8815 mL 4.7038 mL
20 mM 0.1411 mL 0.7056 mL 1.4112 mL 3.5279 mL
25 mM 0.1129 mL 0.5645 mL 1.1289 mL 2.8223 mL
30 mM 0.0941 mL 0.4704 mL 0.9408 mL 2.3519 mL
40 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7639 mL
50 mM 0.0564 mL 0.2822 mL 0.5645 mL 1.4112 mL
60 mM 0.0470 mL 0.2352 mL 0.4704 mL 1.1760 mL
80 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8820 mL
100 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.7056 mL
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SPHINX Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SPHINX848057-98-7SRPKSerine-arginine protein kinasesscaffoldSRSFEGFphosphorylationGAPDHsmall moleculeVEGFmiceratAMDInhibitorinhibitorinhibit

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