SRD5A1-IN-1
Based on 1 Customer Validation
SRD5A1-IN-1 (Compound 4) is a competitive and covalent steroid 5α-reductase type 1 (SRD5A1) inhibitor with an IC50 of 1.44 µM. SRD5A1-IN-1 modulates SRD5A1 function, leading to a lower level of dihydrotestosterone (DHT) production and SRD5A1 protein suppression.
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 2279077-93-7
- Formula: C17H11F6NO3
- Molecular Weight:391.26
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Metabolism/Protease Compound Library
HY-L012
-
Covalent Screening Library
HY-L036
-
Endocrinology Compound Library
HY-L047
-
Metabolic Enzyme Compound Library
HY-L146
-
Cysteine Targeted Covalent Library
HY-L153
-
Bioactive Compound Library Max
HY-L181
-
Covalent Screening Library Plus
HY-L036P
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 1.44 µM (SRD5A1)[1]
SRD5A1-IN-1 (Compound 4) (0.5-2.5 μM; 24 h) decreases SRD5A1 protein expression[1].
SRD5A1-IN-1 (0-2.5 μM; 12 h) modulates SRD5A1 via the dual actions that affect the level of SRD5A1 protein expression and the activity of the SRD5A1 enzyme[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HaCaT
-
Concentration:0.5, 1, and 2.5 µM
-
Incubation Time:12 h and 24 h
-
Result:Showed a significant decrease in SRD5A1 protein expression at 1 and 2.5 µM at 24 h, whereas there were no significant changes in the level of SRD5A1 protein at 12 h.
-
Cell Line:HaCaT
-
Concentration:0.5, 1, and 2.5 µM
-
Incubation Time:12 h and 24 h
-
Result:Did not affect the mRNA expression of SRD5A1 at both incubation times.
-
Cell Line:HaCaT
-
Concentration:0.2, 0.5, 1, and 2.5 µM
-
Incubation Time:24 h
-
Result:Displayed no significant cytotoxicity (IC50: 29.99 ± 8.69 µM).
Chemical Information
-
CAS No. 2279077-93-7
-
Appearance Solid
-
Molecular Weight 391.26
-
Formula C17H11F6NO3
-
Color White to off-white
-
SMILES
O=C(NC1=CC(C(F)(F)F)=CC(C(F)(F)F)=C1)/C=C/C2=CC(O)=C(C=C2)O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (255.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (272 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5558 mL | 12.7792 mL | 25.5585 mL | 63.8961 mL |
| 5 mM | 0.5112 mL | 2.5558 mL | 5.1117 mL | 12.7792 mL | |
| 10 mM | 0.2556 mL | 1.2779 mL | 2.5558 mL | 6.3896 mL | |
| 15 mM | 0.1704 mL | 0.8519 mL | 1.7039 mL | 4.2597 mL | |
| 20 mM | 0.1278 mL | 0.6390 mL | 1.2779 mL | 3.1948 mL | |
| 25 mM | 0.1022 mL | 0.5112 mL | 1.0223 mL | 2.5558 mL | |
| 30 mM | 0.0852 mL | 0.4260 mL | 0.8519 mL | 2.1299 mL | |
| 40 mM | 0.0639 mL | 0.3195 mL | 0.6390 mL | 1.5974 mL | |
| 50 mM | 0.0511 mL | 0.2556 mL | 0.5112 mL | 1.2779 mL | |
| 60 mM | 0.0426 mL | 0.2130 mL | 0.4260 mL | 1.0649 mL | |
| 80 mM | 0.0319 mL | 0.1597 mL | 0.3195 mL | 0.7987 mL | |
| 100 mM | 0.0256 mL | 0.1278 mL | 0.2556 mL | 0.6390 mL |
SRD5A1-IN-1 Related Classifications
HY-152094 Related Classifications
Powered by Bioz