Tetrazine-biotin

Name: Tetrazine-biotin
Brand: MedChemExpress
SKU: HY-136095
Rating: 4.5 (1 reviews)

Cat. No.: HY-136095 Purity: 95.87%: FAQs
Data Sheet SDS COA Handling Instructions

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Tetrazine-biotin is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Tetrazine-biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-biotin Chemical Structure

CAS No. : 1714123-51-9

Size	Price	Stock	Quantity
5 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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View All Isoforms

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetrazine-biotin is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs)^[1]. Tetrazine-biotin is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Cleavable Linker

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

413.50

Formula

C₁₉H₂₃N₇O₂S

CAS No.

1714123-51-9

Appearance

Solid

Color

Purple to purplish red

SMILES

O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)CCCC[C@@H]3SC[C@]([C@]3([H])N4)([H])NC4=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (120.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4184 mL	12.0919 mL	24.1838 mL
5 mM	0.4837 mL	2.4184 mL	4.8368 mL
10 mM	0.2418 mL	1.2092 mL	2.4184 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.87%

Select Batch:

Data Sheet (269 KB) SDS (392 KB)

COA (246 KB) HNMR (262 KB) RP-HPLC (233 KB) MS (79 KB)

Handling Instructions (2659 KB)

References

[1]. Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4184 mL	12.0919 mL	24.1838 mL	60.4595 mL
	5 mM	0.4837 mL	2.4184 mL	4.8368 mL	12.0919 mL
	10 mM	0.2418 mL	1.2092 mL	2.4184 mL	6.0459 mL
	15 mM	0.1612 mL	0.8061 mL	1.6123 mL	4.0306 mL
	20 mM	0.1209 mL	0.6046 mL	1.2092 mL	3.0230 mL
	25 mM	0.0967 mL	0.4837 mL	0.9674 mL	2.4184 mL
	30 mM	0.0806 mL	0.4031 mL	0.8061 mL	2.0153 mL
	40 mM	0.0605 mL	0.3023 mL	0.6046 mL	1.5115 mL
	50 mM	0.0484 mL	0.2418 mL	0.4837 mL	1.2092 mL
	60 mM	0.0403 mL	0.2015 mL	0.4031 mL	1.0077 mL
	80 mM	0.0302 mL	0.1511 mL	0.3023 mL	0.7557 mL
	100 mM	0.0242 mL	0.1209 mL	0.2418 mL	0.6046 mL