Thonningianin B
Based on 1 Customer Validation
Thonningianin B is an antioxidant and an autophagy enhancer.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 271579-12-5
- Formula: C35H30O17
- Molecular Weight:722.60
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Thonningianin B shows strong scavenging action against the DPPH radical. The DPPH radical is scavenged completely by a 34.5 µM solution of Thonningianin B. In a separate experiment, an IC50 value of Thonningianin B is determined to be 21 μM[1].
Thonningianin B (0-100 μM; 24 h) inhibits BV-2 cell viability with an IC50 of 46.74 μM[2].
Thonningianin B (10 μM; 24 h) significantly improves the ratio of LC3-II/LC3-I and the average number of GFP-LC3 puncta per cell in BV-2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BV-2 cells
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Concentration:0-100 μM
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Incubation Time:24 h
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Result:Inhibited viability with an IC50 of 46.74 μM.
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Cell Line:BV-2 cells
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Concentration:10 μM
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Incubation Time:24 h
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Result:Significantly improved the ratio of LC3-II/LC3-I and the average number of GFP-LC3 puncta per cell.
Chemical Information
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CAS No. 271579-12-5
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Appearance Solid
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Molecular Weight 722.60
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Formula C35H30O17
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Color Off-white to light brown
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SMILES
O=C(C1=C(O)C=C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](COC3=O)O2)OC(C4=CC(O)=C(O)C(O)=C4C5=C(O)C(O)=C(O)C=C53)=O)O)O)C=C1O)CCC6=CC=CC=C6
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 66.67 mg/mL (92.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Methanol : 50 mg/mL (69.19 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.73 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (1.73 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ohtani II, et al. Thonningianins A and B, new antioxidants from the African medicinal herb Thonningia sanguinea. J Nat Prod. 2000 May;63(5):676-9. [Content Brief]
[2]. Zhou XG, et al. Targeting microglial autophagic degradation of the NLRP3 inflammasome for identification of thonningianin A in Alzheimer's disease. Inflamm Regen. 2022 Aug 3;42(1):25. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Methanol / DMSO | 1 mM | 1.3839 mL | 6.9195 mL | 13.8389 mL | 34.5973 mL |
| 5 mM | 0.2768 mL | 1.3839 mL | 2.7678 mL | 6.9195 mL | |
| 10 mM | 0.1384 mL | 0.6919 mL | 1.3839 mL | 3.4597 mL | |
| 15 mM | 0.0923 mL | 0.4613 mL | 0.9226 mL | 2.3065 mL | |
| 20 mM | 0.0692 mL | 0.3460 mL | 0.6919 mL | 1.7299 mL | |
| 25 mM | 0.0554 mL | 0.2768 mL | 0.5536 mL | 1.3839 mL | |
| 30 mM | 0.0461 mL | 0.2306 mL | 0.4613 mL | 1.1532 mL | |
| 40 mM | 0.0346 mL | 0.1730 mL | 0.3460 mL | 0.8649 mL | |
| 50 mM | 0.0277 mL | 0.1384 mL | 0.2768 mL | 0.6919 mL | |
| 60 mM | 0.0231 mL | 0.1153 mL | 0.2306 mL | 0.5766 mL | |
| DMSO | 80 mM | 0.0173 mL | 0.0865 mL | 0.1730 mL | 0.4325 mL |
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