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TRFS-green 

Cat. No.: HY-115640
Handling Instructions

TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm.

For research use only. We do not sell to patients.

TRFS-green Chemical Structure

TRFS-green Chemical Structure

CAS No. : 1513848-14-0

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Description

TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm[1].

In Vitro

TRFS-green itself emits at ∼480 nm when excited at 377 nm[1].
TRFS-green displays a green fluorescence off−on change induced by the TrxR-mediated disulfide cleavage and subsequent intramolecular cyclization to liberate the masked naphthalimide fluorophore[1].
The fluorescence signal of TRFS-green in living cells was inhibited by pretreating the cells with TrxR inhibitor in a dose-dependent manner, potentiating the development of living cell-based screening assay for identifying TrxR inhibitors[1].
After incubating the cells with TRFS-green (10 μM) for 4 h, the bright green fluorescence appears[1].
TRFS-green shows no response to GSH or Cys, the predominant thiols in cells, or the endogenous reducing agents, such as vitamin C and NADPH[1].
TRFS-green is the first fluorescent probe of TrxR with a green emission. However, the response rate of TRFS-green to the enzyme is slow, and it takes more than 2 h to reach the maximal fluorescence signal even using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

416.51

Formula

C₂₀H₂₀N₂O₄S₂

CAS No.

1513848-14-0

SMILES

O=C(OC1CSSC1)NC2=CC=C(C3=C4C=CC=C32)C(N(CCCC)C4=O)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

TRFS-greenOthersthioredoxinreductaseTrxRTCEPgreenInhibitorinhibitorinhibit

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