Calcein-AM (solution)

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.Preparation of Calcein-AM working solution
Dilute the stock solution in serum-free cell culture medium or PBS. The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: Please adjust the concentration of Calcein AM working solution according to the actual situation.

2.Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10⁶/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 30-45 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or fluorescence microplate readers.
3.Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-45 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or fluorescence microplate readers.
4. Precautions
4.1 Please adjust the concentration of Calcein-AM working solution according to the actual situation.
4.2 This product is for R&D use only, not for drug, household, or other uses.
4.3 For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.