Junceellolide C
Junceellolide C is a transcription inhibitor of cccDNA. Junceellolide C inhibits HBV DNA replication and significantly decreases the level of supernatant HBV RNA with EC50 values of 5.19, 3.52 μM respectively in HepAD38 cells. Junceellolide C is a potent anti-HBV agent.
For research use only. We do not sell to patients.
- CAS No.: 121769-82-2
- Formula: C26H33ClO10
- Molecular Weight:540.99
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
EC50: 2.27 μM (HBV DNA replication), 3.52 μM (HBV RNA decrease) in HBeAg cells[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BMDM | CC50 |
>50 μM
Compound: 31
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Cytotoxicity against C57BL/6 mouse BMDM cells incubated for 48 hrs by MTT assay
Cytotoxicity against C57BL/6 mouse BMDM cells incubated for 48 hrs by MTT assay
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[PMID: 36446206] |
| HepG2 | CC50 |
>100 μM
Compound: 27
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Cytotoxicity against human HepG2 cells assessed as cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell viability incubated for 72 hrs by MTT assay
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[PMID: 32690259] |
In Vitro
Junceellolide C (10 μM; 72 hours) inhibits HBV DNA replication and significantly decreases the level of supernatant HBV RNA in HepAD38 cells with EC50 values of 2.27, 3.52 μM, respectively[1].
Junceellolide C (10 μM; 8 days) can capable to inhibit HBV cccDNA replenishment and also to induce the existed HBV cccDNA degradation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HepAD38 cells
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Concentration:10 μM
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Incubation Time:72 h, 8 days
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Result:Inhibited cccDNA transcription.
Chemical Information
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CAS No. 121769-82-2
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Molecular Weight 540.99
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Formula C26H33ClO10
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SMILES
C[C@@]12[C@]([C@@H]([C@]3([C@@]([C@H](C(/C=C\[C@@H]2OC(C)=O)=C)Cl)([H])OC([C@@H]3C)=O)O)OC(C)=O)([H])[C@]4(CC[C@@H]1OC(C)=O)CO4
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Structure Classification
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Initial Source
South China Sea gorgonian Dichotella gemmacea
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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PCNA Immunodetection Proliferation Assay
PCNA immunodetection measures proliferative activity by detecting proliferating cell nuclear antigen, a nuclear protein associated with DNA polymerase δ function and DNA replication. The assay readout is the proportion of PCNA-positive nuclei among total counted cells, but PCNA labeling is not identical to BrdU labeling because PCNA can mark late G1/early S-associated replication competence and may persist beyond active DNA synthesis depending on fixation and extraction conditions.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Junceellolide C Related Classifications
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