X-alpha-Gal
Based on 1 Customer Validation
X-alpha-Gal is a chromogenic substrate that is used to screen colonies with high activity of β-galactopyranoside. X-alpha-Gal also detects α-galactosidase activity. X-alpha-Gal can be used in yeast two hybrid screening for MEL1 gene activation. X-alpha-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 107021-38-5
- Formula: C14H15BrClNO6
- Molecular Weight:408.63
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Biological Activity
Chemical Information
-
CAS No. 107021-38-5
-
Appearance Solid
-
Molecular Weight 408.63
-
Formula C14H15BrClNO6
-
Color White to light yellow
-
SMILES
O[C@@H]1[C@@H](O)[C@@H](O)[C@H](O[C@@H]1OC2=CNC3=C2C(Cl)=C(C=C3)Br)CO
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (244.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[2]. Rac, A., & Fulgosi, H. (2019). Qualitative and quantitative dataset of TROL protein interaction with C3 and C4 ferredoxin: NADP+ oxidoreductases. Data in brief, 28, 105038. [Content Brief]
[3]. Aho, S., et al., (1997). A novel reporter gene MEL1 for the yeast two-hybrid system. Analytical biochemistry, 253(2), 270–272. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4472 mL | 12.2360 mL | 24.4720 mL | 61.1800 mL |
| 5 mM | 0.4894 mL | 2.4472 mL | 4.8944 mL | 12.2360 mL | |
| 10 mM | 0.2447 mL | 1.2236 mL | 2.4472 mL | 6.1180 mL | |
| 15 mM | 0.1631 mL | 0.8157 mL | 1.6315 mL | 4.0787 mL | |
| 20 mM | 0.1224 mL | 0.6118 mL | 1.2236 mL | 3.0590 mL | |
| 25 mM | 0.0979 mL | 0.4894 mL | 0.9789 mL | 2.4472 mL | |
| 30 mM | 0.0816 mL | 0.4079 mL | 0.8157 mL | 2.0393 mL | |
| 40 mM | 0.0612 mL | 0.3059 mL | 0.6118 mL | 1.5295 mL | |
| 50 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2236 mL | |
| 60 mM | 0.0408 mL | 0.2039 mL | 0.4079 mL | 1.0197 mL | |
| 80 mM | 0.0306 mL | 0.1530 mL | 0.3059 mL | 0.7648 mL | |
| 100 mM | 0.0245 mL | 0.1224 mL | 0.2447 mL | 0.6118 mL |