Vari Fluor 488-Streptavidin
Based on 1 publication(s) in Google Scholar
Vari Fluor 488-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=490 nm/515 nm.
Product Concentration: 0.8 mg/mL
For research use only. We do not sell to patients.
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Storage:
Solution, -20°C, protect from light, 2 years
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Vari Fluor 488-Streptavidin
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Biological Activity
Product Concentration: 0.8 mg/mL
General Protocol
1.Protein treatment
Before use, centrifuge at 5000× g for 3 min, only the supernatant is used for experiments to eliminate protein aggregates and reduce non-specific background staining.
2.Labeling
Dilute Vari Fluor-Streptavidin at 1:500-1:1000, which can be adjusted according to the specific conditions of the protein or antibody.
Storage
-20°C,
Protect from light
Precautions
1. The actual content of the dye is small, dissolve it directly in the tube after receiving it for experiments.
2. Before use, please centrifuge the product to the bottom of the tube instantaneously before subsequent experiments.
3. This product is intended for scientific research only by professionals and is not to be used for clinical diagnosis or treatment, food or medicine.
4. For your safety and health, please wear lab coat and disposable gloves.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
488
Chemical Information
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Appearance Liquid
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Color Light yellow to yellow
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SMILES
[Vari Fluor 488-Streptavidin]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, protect from light, 2 years
Publications (1)
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Journal Impact Factor
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Most Recent
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Sci Adv
2026 Feb 20;12(8):eaeb9574. PMID: 41719385
Purity & Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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