WRN inhibitor 8
Based on 1 Customer Validation
WRN inhibitor 8 is an inhibitor of WRN helicase with an IC50 value of 48 nM. WRN inhibitor 8 can inhibit the proliferation of tumor cells and be used in the study of cancer.
For research use only. We do not sell to patients.
- Purity : 98.07%
- CAS No.: 3026502-95-1
- Formula: C22H23F2N3O4S
- Molecular Weight:463.50
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
IC50 & Target
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Helicase |
In Vitro
WRN inhibitor 8 (Example 224) (0-100 μM; 5 days) can inhibit the proliferation of HCT-116 and SW480 cells, with GI50 values of 0.01699 μM and 13.64 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3026502-95-1
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Appearance Solid
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Molecular Weight 463.50
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Formula C22H23F2N3O4S
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Color White to off-white
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SMILES
CS(/C=C/[C@H](C1CC1)NC(C(C(OC2=CC=CC=C2)=N3)=CN=C3C(F)(C4CC4)F)=O)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (269.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1575 mL | 10.7875 mL | 21.5750 mL | 53.9374 mL |
| 5 mM | 0.4315 mL | 2.1575 mL | 4.3150 mL | 10.7875 mL | |
| 10 mM | 0.2157 mL | 1.0787 mL | 2.1575 mL | 5.3937 mL | |
| 15 mM | 0.1438 mL | 0.7192 mL | 1.4383 mL | 3.5958 mL | |
| 20 mM | 0.1079 mL | 0.5394 mL | 1.0787 mL | 2.6969 mL | |
| 25 mM | 0.0863 mL | 0.4315 mL | 0.8630 mL | 2.1575 mL | |
| 30 mM | 0.0719 mL | 0.3596 mL | 0.7192 mL | 1.7979 mL | |
| 40 mM | 0.0539 mL | 0.2697 mL | 0.5394 mL | 1.3484 mL | |
| 50 mM | 0.0431 mL | 0.2157 mL | 0.4315 mL | 1.0787 mL | |
| 60 mM | 0.0360 mL | 0.1798 mL | 0.3596 mL | 0.8990 mL | |
| 80 mM | 0.0270 mL | 0.1348 mL | 0.2697 mL | 0.6742 mL | |
| 100 mM | 0.0216 mL | 0.1079 mL | 0.2157 mL | 0.5394 mL |
WRN inhibitor 8 Related Classifications
HY-160859 Related Classifications
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