Zenidolol hydrochloride
Based on 47 publication(s) in Google Scholar
Zenidolol (ICI-118551) hydrochloride is a highly selective β2 adrenergic receptor antagonist, with Kis of 0.7, 49.5 and 611 nM for β2, β1 and β3 receptors, respectively.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 72795-01-8
- Formula: C17H28ClNO2
- Molecular Weight:313.86
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications Citing Use of MedChemExpress (MCE) Zenidolol hydrochloride
More- Cancer Cell. 2026 Jun 18:S1535-6108(26)00263-1. [Abstract]
- Immunity. 2026 Mar 10;59(3):598-617.e11. [Abstract]
- Cancer Discov. 2025 Jan 13;15(1):202-226. [Abstract]
- Cancer Res. 2025 Dec 31. [Abstract]
- Nat Commun. 2024 Nov 28;15(1):10361. [Abstract]
- Nat Commun. 2023 May 2;14(1):2523. [Abstract]
- Nat Commun. 2021 Nov 26;12(1):6937. [Abstract]
- Nat Commun. 2020 Sep 25;11(1):4857. [Abstract]
- J Exp Clin Cancer Res. 2019 Apr 25;38(1):174. [Abstract]
- Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
- Adv Sci (Weinh). 2024 Nov;11(41):e2402393. [Abstract]
- Sci Adv. 2026 Mar 6;12(10):eaea7017. [Abstract]
- Sci Adv. 2024 Jul 19;10(29):eadp5239. [Abstract]
- Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
- J Exp Med. 2023 Nov 6;220(11):e20230577. [Abstract]
- J Pharm Anal. 2024 Jul;14(7):100934. [Abstract]
- Diabetes. 2022 May 1;71(5):921-933. [Abstract]
- Chin Med J (Engl). 2026 Apr 23. [Abstract]
- Int J Nanomedicine. 2025 May 12:20:6023-6041. [Abstract]
- Cell Rep. 2025 Sep 23;44(10):116337. [Abstract]
- Br J Pharmacol. 2020 Jan;177(2):282-297. [Abstract]
- Anal Chem. 2026 Feb 17;98(6):4895-4904. [Abstract]
- Commun Biol. 2022 Apr 8;5(1):339. [Abstract]
- Commun Biol. 2021 Dec 3;4(1):1359. [Abstract]
- J Endocrinol. 2023 Feb 16;256(3):e220335. [Abstract]
- Eur J Pharmacol. 2022 Aug 5:928:175110. [Abstract]
- Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):13. [Abstract]
- Int Dent J. 2025 Apr;75(2):807-816. [Abstract]
- J Nutr Biochem. 2018 Aug:58:110-118. [Abstract]
- Oncol Rep. 2016 Sep;36(3):1576-84. [Abstract]
- Mol Oncol. 2022 Aug;16(15):2881-2895. [Abstract]
- BMC Cancer. 2019 Nov 26;19(1):1142. [Abstract]
- Neuroscience. 2019 Mar 1:401:59-72. [Abstract]
- Pflugers Arch. 2021 Dec;473(12):1899-1910. [Abstract]
- Biochem Biophys Res Commun. 2024 Apr 9:703:149689. [Abstract]
- J Oral Maxillofac Surg. 2020 Oct;78(10):1871.e1-1871.e23. [Abstract]
- BMC Ophthalmol. 2021 Dec 5;21(1):419. [Abstract]
- bioRxiv. 2025 Jul 26:2025.07.22.666009. [Abstract]
- Environ Toxicol. 2024 Mar;39(3):1682-1699. [Abstract]
- bioRxiv. 2024 Feb 19.
- Biomed Pharmacother. 2023 May:161:114523. [Abstract]
- Research Square Preprint. 2022 Mar.
- Research Square Preprint. 2021 Nov.
- Research Square Preprint. 2021 Sep.
- Research Square Preprint. 2021 Feb.
- Research Square Preprint. 2020 Oct.
- bioRxiv. 2020 Jan.
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Cell Proliferation/Viability Assay
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IF
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WB
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Cell Imaging/Staining
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WB
All Adrenergic Receptor Isoforms
More
Biological Activity
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β adrenergic receptor |
Zenidolol (ICI-118551) hydrochloride inhibits cAMP accumulation with IC50 of 1.7 μM in IMCD cells[1].
Zenidolol (ICI-118551; 10 μM) hydrochloride induces a prominent vasorelaxation of norepinephrine (NE)-precontracted PA but not AO[2].
In failing human heart, Zenidolol (ICI-118551) hydrochloride has significant effects on beat duration, with time-to-peak contraction and time-to-90% relaxation reduced compared with basal contraction. Negative Inotropic Effect of Zenidolol (ICI-118551) hydrochloride Is Not cAMP-Related. Overexpression of β2AR in rabbit myocytes enhances negative inotropic effects of Zenidolol (ICI-118551) hydrochloride[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 72795-01-8
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Appearance Solid
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Molecular Weight 313.86
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Formula C17H28ClNO2
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Color White to light yellow
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SMILES
CC1=C(CCC2)C2=C(OC[C@H](O)[C@@H](C)NC(C)C)C=C1.[H]Cl
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Synonyms
ICI-118551 hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (47)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Macrophage-mediated brain-bone marrow crosstalk promotes chronic stress-induced glioma growth. [Abstract]2026 Jun 18:S1535-6108(26)00263-1. PMID: 42314665 -
Immunity
2026 Mar 10;59(3):598-617.e11. PMID: 41722568 -
Cancer Discov
Neuro-mesenchymal interaction mediated by a β2 adrenergic-nerve growth factor feedforward loop promotes colorectal cancer progression. [Abstract]2025 Jan 13;15(1):202-226. PMID: 39137067 -
Cancer Res
2025 Dec 31. PMID: 41474982 -
Nat Commun
A brain-to-liver signal mediates the inhibition of liver regeneration under chronic stress in mice. [Abstract]2024 Nov 28;15(1):10361. PMID: 39609433 -
Nat Commun
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice. [Abstract]2023 May 2;14(1):2523. PMID: 37130842 -
Nat Commun
Fat mass and obesity-associated protein regulates RNA methylation associated with depression-like behavior in mice. [Abstract]2021 Nov 26;12(1):6937. PMID: 34836959 -
Nat Commun
DeSiphering receptor core-induced and ligand-dependent conformational changes in arrestin via genetic encoded trimethylsilyl 1H-NMR probe. [Abstract]2020 Sep 25;11(1):4857. PMID: 32978402 -
J Exp Clin Cancer Res
Mammalian Eps15 homology domain 1 potentiates angiogenesis of non-small cell lung cancer by regulating β2AR signaling. [Abstract]2019 Apr 25;38(1):174. PMID: 31023336 -
Adv Sci (Weinh)
Norepinephrine Induces Sertoli Cell Ferroptosis via Receptors Desensitization Causing Stress-Related Male Reproductive Dysfunction. [Abstract]2025 Oct 6:e04817. PMID: 41051274
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
Zenidolol (ICI118551; ICI) (1 µM; 24-72 H) reversed the NE‐induced reduction in Sertoli cell viability.
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
Zenidolol (ICI118551; ICI) (1 µM) reversed NE-induced ACSL4 increase and GPX4 decrease in Sertoli cells.
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
Zenidolol (ICI118551; ICI) (1 µM) reversed NE-induced alterations in the expression of SLC7A11, GPX4, and ACSL4 in TM4 cells.
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
Zenidolol (ICI118551; ICI) (1 µM) alleviated the NE-induced increase in death of TM4 cells.
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 6:e04817. [Abstract]
Zenidolol (ICI118551; ICI) (1 µM) normalized tight junction markers ZO-1 and Claudin-11 protein levels in TM4 cells suppressed by NE.
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Adv Sci (Weinh)
Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling. [Abstract]2024 Nov;11(41):e2402393. PMID: 39229959 -
Sci Adv
Sympathetic nerve aggravates autoimmune skin disease via NE-adrenergic receptor axis: Neuroimmune cross-talk insights from vitiligo. [Abstract]2026 Mar 6;12(10):eaea7017. PMID: 41779853 -
Sci Adv
Beta2 adrenergic receptor-mediated abnormal myelopoiesis drives neuroinflammation in aged patients with traumatic brain injury. [Abstract]2024 Jul 19;10(29):eadp5239. PMID: 39028822 -
Int J Biol Sci
Chronic stress promotes colorectal cancer progression by enhancing glycolysis through β2-AR/CREB1 signal pathway. [Abstract]2023 Apr 2;19(7):2006-2019. PMID: 37151872
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2023 Apr 2;19(7):2006-2019. [Abstract]
Zenidolol (ICI; 10 μM; 2 h) abolishes the Epinephrine-induced phosphorylation of CREB1 in MC38 and HT-29 cells.
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J Exp Med
2023 Nov 6;220(11):e20230577. PMID: 37584653 -
J Pharm Anal
Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptor. [Abstract]2024 Jul;14(7):100934. PMID: 39139999 -
Diabetes
Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity Via Inhibiting USP33-Mediated ADRB2 Deubiquitination. [Abstract]2022 May 1;71(5):921-933. PMID: 35192681 -
Chin Med J (Engl)
Skin-localized sympathetic nerve-keratinocyte crosstalk fuels psoriatic inflammation via neutrophil recruitment. [Abstract]2026 Apr 23. PMID: 42036893 -
Int J Nanomedicine
Bupivacaine Nanoparticles Inhibit Triple-Negative Breast Tumor Growth by Suppressing the Noradrenergic Nerves in Tumor Microenvironment. [Abstract]2025 May 12:20:6023-6041. PMID: 40385493 -
Cell Rep
2025 Sep 23;44(10):116337. PMID: 40991929 -
Br J Pharmacol
Pharmacological targeting of β2 -adrenoceptors is neuroprotective in the LPS inflammatory rat model of Parkinson's disease. [Abstract]2020 Jan;177(2):282-297. PMID: 31506926 -
Anal Chem
An Allosteric Assay for Identifying Ligands Binding to β2 Adrenergic Receptor by Surface-Enhanced Raman Scattering (SERS)-Active Nanoparticles. [Abstract]2026 Feb 17;98(6):4895-4904. PMID: 41637639 -
Commun Biol
Suppression of trabecular meshwork phagocytosis by norepinephrine is associated with nocturnal increase in intraocular pressure in mice. [Abstract]2022 Apr 8;5(1):339. PMID: 35396348 -
Commun Biol
Reserpine improves Enterobacteriaceae resistance in chicken intestine via neuro-immunometabolic signaling and MEK1/2 activation. [Abstract]2021 Dec 3;4(1):1359. PMID: 34862463 -
J Endocrinol
2023 Feb 16;256(3):e220335. PMID: 36633355 -
Eur J Pharmacol
Dopamine regulates astrocytic IL-6 expression and process formation via dopamine receptors and adrenoceptors. [Abstract]2022 Aug 5:928:175110. PMID: 35738452 -
Invest Ophthalmol Vis Sci
Activation of Sympathetic Nervous System Drives Dry Eye Onset Via Norepinephrine-β2-Adrenergic Receptor Signaling in Mice. [Abstract]2025 Jun 2;66(6):13. PMID: 40465266 -
Int Dent J
2025 Apr;75(2):807-816. PMID: 39043526 -
J Nutr Biochem
Genistein rescues hypoxia-induced pulmonary arterial hypertension through estrogen receptor and β-adrenoceptor signaling. [Abstract]2018 Aug:58:110-118. PMID: 29886191
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: J Nutr Biochem. 2018 Aug:58:110-118. [Abstract]
PASMCs are pretreated with or not Norepinephrine (50 μM) or ICI 118,551 (5 nM), and α-SMA (D) are analyzed.
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Oncol Rep
2016 Sep;36(3):1576-84. PMID: 27432558
Zenidolol hydrochloride purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2016 Sep;36(3):1576-84. [Abstract]
Western blotting of Bcl-2 expression in 8505C lysates after in vitro pharmacologic interventions using propranolol and ICI118551 for 24 h. Western blotting of AKT, p-AKT, mTOR, p-mTOR in 8505C lysates after in vitro pharmacologic interventions using propranolol and ICI118551for 24 h.
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Mol Oncol
NNK from tobacco smoking enhances pancreatic cancer cell stemness and chemoresistance by creating a β2AR-Akt feedback loop that activates autophagy. [Abstract]2022 Aug;16(15):2881-2895. PMID: 35593085 -
BMC Cancer
β2AR-HIF-1α-CXCL12 signaling of osteoblasts activated by isoproterenol promotes migration and invasion of prostate cancer cells. [Abstract]2019 Nov 26;19(1):1142. PMID: 31771535 -
Neuroscience
Natural Killer Cells may Exert Antidepressant-like Effects in Mice by Controlling the Release of Inflammatory Factors. [Abstract]2019 Mar 1:401:59-72. PMID: 30641114 -
Pflugers Arch
Increased β-adrenergic stimulation augments vascular smooth muscle cell calcification via PKA/CREB signalling. [Abstract]2021 Dec;473(12):1899-1910. PMID: 34564739 -
Biochem Biophys Res Commun
Olodaterol promotes thermogenesis in brown adipocytes via regulation of the β2-AR/cAMP/PKA signaling pathway. [Abstract]2024 Apr 9:703:149689. PMID: 38382361 -
J Oral Maxillofac Surg
Overexpression of β-Adrenergic Receptors and the Suppressive Effect of β2-Adrenergic Receptor Blockade in Oral Squamous Cell Carcinoma. [Abstract]2020 Oct;78(10):1871.e1-1871.e23. PMID: 32640209 -
BMC Ophthalmol
β-blocker eye drops affect ocular surface through β2 adrenoceptor of corneal limbal stem cells. [Abstract]2021 Dec 5;21(1):419. PMID: 34863129 -
bioRxiv
2025 Jul 26:2025.07.22.666009. PMID: 40777309 -
Environ Toxicol
Phenylacetyl glutamine (PAGln) enhances cardiomyocyte death after myocardial infarction through β1 adrenergic receptor. [Abstract]2024 Mar;39(3):1682-1699. PMID: 38041472 -
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Biomed Pharmacother
Interference of sympathetic overactivation restores limbal stem/progenitor cells function and accelerates corneal epithelial wound healing in diabetic mice. [Abstract]2023 May:161:114523. PMID: 36931034 -
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Solvent & Solubility
DMSO : 25 mg/mL (79.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 8.33 mg/mL (26.54 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 1 mg/mL (3.19 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
One hour prior to assay, the growth media are removed from the wells and replaced with 50 uL of Hanks’balanced salt solution that also contained 0.5 mM of MgCl2•6H2O, 0.4 mM of MgSO4•7H2O, 20 mM of N-2-hydroxyethylpiperazine-N’-2ethanesulfonic acid (HEPES), 1.2 mM of 3-isobutyl-1-methylxanthine (IBMX), 0.95 mM of CaCl2, and 0.05% of BSA. Each plate is placed in a 37°C shaking water bath for dose-response studies. In one study, various doses of isoproterenol (10-9-10-5 M) and β1- and β2-receptor-selective partial agonists (tazolol, prenalterol, salbutamol, and terbutaline, 10-6 and 10-5 M, respectively) are added (5 wells/dose/plate) and incubated for 10 min. In another study, the cells are stimulated with 10 μM isoproterenol in the presence or absence of various doses of β-adrenoceptor antagonists. The incubations are terminated after 10 min by the addition of 100 μL of 10% trichloroacetic acid (TCA) (final TCA concentration of 5%). TCA is removed twice by extraction with H20-saturated ether, and samples are dried at 80°C overnight, prior to resuspension in 50 mM of sodium acetate buffer. The CAMP content is measured with a radioimmunoassay kit.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Yasuda G, et al. The beta 1- and beta 2-adrenoceptor subtypes in cultured rat inner medullary collecting duct cells. Am J Physiol. 1996 Sep;271(3 Pt 2):F762-9. [Content Brief]
[2]. Wenzel D, et al. beta(2)-adrenoceptor antagonist ICI 118,551 decreases pulmonary vascular tone in mice via a G(i/o) protein/nitric oxide-coupled pathway. Hypertension. 2009 Jul;54(1):157-63. [Content Brief]
[3]. Gong H, et al. Specific beta(2)AR blocker ICI 118,551 actively decreases contraction through a G(i)-coupled form of the beta(2)AR in myocytes from failing human heart. Circulation. 2002 May 28;105(21):2497-503. [Content Brief]
[4]. Hoffmann C, et al. Comparative pharmacology of human beta-adrenergic receptor subtypes--characterization of stably transfected receptors in CHO cells. Naunyn Schmiedebergs Arch Pharmacol. 2004 Feb;369(2):151-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.1861 mL | 15.9307 mL | 31.8613 mL | 79.6534 mL |
| 5 mM | 0.6372 mL | 3.1861 mL | 6.3723 mL | 15.9307 mL | |
| 10 mM | 0.3186 mL | 1.5931 mL | 3.1861 mL | 7.9653 mL | |
| 15 mM | 0.2124 mL | 1.0620 mL | 2.1241 mL | 5.3102 mL | |
| 20 mM | 0.1593 mL | 0.7965 mL | 1.5931 mL | 3.9827 mL | |
| 25 mM | 0.1274 mL | 0.6372 mL | 1.2745 mL | 3.1861 mL | |
| DMSO | 30 mM | 0.1062 mL | 0.5310 mL | 1.0620 mL | 2.6551 mL |
| 40 mM | 0.0797 mL | 0.3983 mL | 0.7965 mL | 1.9913 mL | |
| 50 mM | 0.0637 mL | 0.3186 mL | 0.6372 mL | 1.5931 mL | |
| 60 mM | 0.0531 mL | 0.2655 mL | 0.5310 mL | 1.3276 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.