ZHO-197 

ZHO-197 is a dual bacterial peptide deformylase and methionine aminopeptidase inhibitor with antibacterial activity. ZHO-197 exhibits an Escherichia coli peptide deformylase IC₅₀ of 0.021 μM and a human peptide deformylase IC₅₀ of 3.289 μM. ZHO-197 displays broad-spectrum antibacterial activity against gram-positive and gram-negative bacterial strains. ZHO-197 can be used for the research of bacterial infection^[1].

ZHO-197 (0.0033-10 μM; 3 h) potently inhibits recombinant Escherichia coli peptide deformylase with an IC₅₀ of 0.021 ± 0.003 μM and shows selective inhibition for bacterial over human PDF^[1].
ZHO-197 (0-256 μg/mL; overnight) exhibits antibacterial activity against gram-positive and gram-negative bacterial strains, with MIC values of 32, 2, 128, 4, and 2 μg/mL against Escherichia coli BW25113, E. coli ΔtolC, E. coli DSM30083, Staphylococcus aureus DSM20231, and Bacillus subtilis 168, respectively, and an MIC₉₀ of 1.87 μg/mL against Mycobacterium tuberculosis Erdman^[1].
ZHO-197 (30-300 μM; 72 h) shows no cytotoxicity against human peripheral blood mononuclear cells^[1].
ZHO-197 (64 μg/mL; overnight) acts as a dual inhibitor, effectively inhibiting peptide deformylase and methionine aminopeptidase in intact Bacillus subtilis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

385.50

C₁₉H₃₅N₃O₅

O=CN(O)C[C@@H](CCCCC)C(N[C@@H](C(C)C)C(N1CCC[C@H]1CO)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bachmann N, et al. Towards selective peptide deformylase inhibitors: A mass spectrometry-based evaluation of in cellulo activity of actinonin and bromoindole derivatives. Eur J Med Chem. 2026;308:118678.  [Content Brief]