2,4-D sodium
Based on 1 publication(s) in Google Scholar
2,4-D (2,4-Dichlorophenoxyacetate) sodium is a selective herbicide that can be used for the control of broad-leaved weeds. 2,4-D sodium salt can induce apoptosis. 2,4-D sodium salt inhibits DNA and protein synthesis, thereby preventing normal plant growth and development.
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- Reinheit : 98.0%
- CAS. Nr.: 2702-72-9
- Formel: C8H5Cl2NaO3
- Molecular Weight:243.02
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Speicherung:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2,4-D sodium
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Biologische Aktivität
Beschreibung
In Vitro
2, 4-D sodium salt (25-200 μM, 72 h) inhibits the cell viability of A549 and WI38 with IC50 values of 126 µM and 115 µM, respectively. By influencing cell cycle, apoptosis was induced[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549, W138
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Concentration:25, 50, 75, 100, 150, 200 μM
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Incubation Time:72 h
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Result:Inhibited cell viability in a dose-dependent manner.
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Cell Line:A549
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Concentration:50, 100, 150 μM
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Incubation Time:72 h
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Result:The G0/G1 population decreased to about 57%, 43% and 31%, respectively.
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Cell Line:A549, W138
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Concentration:100, 150 μM
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Incubation Time:72 h
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Result:The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells.
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Cell Line:A549, W138
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Concentration:100, 150 μM
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Incubation Time:72 h
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Result:Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and pro caspase 3.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult Wistar male rats[3]
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Dosage:100 or 200 mg/kg
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Administration:i.g.
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Result:Decreased the testis , seminal vesicles and prostate relative weight. Decreased the number of spermatozoa and sperm motility. Increased the level of FSH and LH.
Chemical Information
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CAS. Nr. 2702-72-9
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Appearance Solid
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Molecular Weight 243.02
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Formel C8H5Cl2NaO3
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Color White to off-white
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SMILES
O=C(O[Na])COC1=CC=C(Cl)C=C1Cl
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Synonyms
2,4-Dichlorophenoxyacetic acid sodium
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (1)
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Journal Impact Factor
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Most Recent
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (411.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (102.87 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (20.57 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (20.57 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protokoll
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (596 KB)
- English - EN (596 KB)
- Français - FR (596 KB)
- Deutsch - DE (596 KB)
- Norwegian - NO (596 KB)
- Español - ES (596 KB)
- Swedish - SV (596 KB)
- Italian - IT (596 KB)
- Korean - KR (596 KB)
- Portuguese - PT (596 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Germaine KJ, et al. Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid. FEMS Microbiol Ecol. 2006 Aug;57(2):302-10. [Content Brief]
[3]. Marouani N, et al. Effects of oral administration of 2,4-dichlorophenoxyacetic acid (2,4-D) on reproductive parameters in male Wistar rats. Environ Sci Pollut Res Int. 2017 Jan;24(1):519-526. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 4.1149 mL | 20.5744 mL | 41.1489 mL | 102.8722 mL |
| 5 mM | 0.8230 mL | 4.1149 mL | 8.2298 mL | 20.5744 mL | |
| 10 mM | 0.4115 mL | 2.0574 mL | 4.1149 mL | 10.2872 mL | |
| 15 mM | 0.2743 mL | 1.3716 mL | 2.7433 mL | 6.8581 mL | |
| 20 mM | 0.2057 mL | 1.0287 mL | 2.0574 mL | 5.1436 mL | |
| 25 mM | 0.1646 mL | 0.8230 mL | 1.6460 mL | 4.1149 mL | |
| 30 mM | 0.1372 mL | 0.6858 mL | 1.3716 mL | 3.4291 mL | |
| 40 mM | 0.1029 mL | 0.5144 mL | 1.0287 mL | 2.5718 mL | |
| 50 mM | 0.0823 mL | 0.4115 mL | 0.8230 mL | 2.0574 mL | |
| 60 mM | 0.0686 mL | 0.3429 mL | 0.6858 mL | 1.7145 mL | |
| 80 mM | 0.0514 mL | 0.2572 mL | 0.5144 mL | 1.2859 mL | |
| 100 mM | 0.0411 mL | 0.2057 mL | 0.4115 mL | 1.0287 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
2,4-D sodium Related Classifications
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