20-5,14-HEDGE
Based on 1 Customer Validation
20-5,14-HEDGE is a 20-HETE analog. 20-5,14-HEDGE induces cell death in organotypic hippocampal slice cultures (OHSCs). 20-5,14-HEDGE enhances ROS production and reduces hypoxia-reoxygenation (HR)-induced apoptosis in ex vivo rat lung slices. 20-5,14-HEDGE protects against lung ischemic reperfusion injury in rats. 20-5,14-HEDGE can be used for the study of pulmonary vascular signaling and lung injury-related research.
For research use only. We do not sell to patients.
- Purity: 99.28%
- CAS No.: 1092380-14-7
- Formula: C22H39NO4
- Molecular Weight:381.55
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
20-5,14-HEDGE (5-20 μM, 18 h) significantly induces dose-dependent cell death in organotypic hippocampal slice cultures (OHSCs), and the dead cells are mainly neurons[1].
20-5,14-HEDGE (1 μM) significantly increases ROS production in cultured BPAECs[3].
20-5,14-HEDGE (1 μM, 6 h) reduces hypoxia-reoxygenation (HR)-induced apoptosis in ex vivo rat lung slices[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Rat lung slices
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Concentration:1 μM
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Incubation Time:6 h
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Result:Reduced HR-induced apoptosis in ex vivo rat lung slices.
Decreased terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) positive cells.
Blunted increase in caspase-3 activity.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague Dawley rats (200-400 g)[2]
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Dosage:30 mg/kg
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Administration:i.p., once, 60 min before surgery
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Result:Exerted protective effects against surgically-induced ischemia-reperfusion lung injury.
Reduced lung hemorrhage, polymorphonuclear cell infiltration, caspase 3 activity, toll-like receptor 4 and high mobility group box 1 expression.
Chemical Information
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CAS No. 1092380-14-7
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Appearance Solid
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Molecular Weight 381.55
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Formula C22H39NO4
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Color White to off-white
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SMILES
OC(CNC(CCC/C=C\CCCCCCC/C=C\CCCCCO)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (262.09 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Han R, et al. 20-HETE Participates in Intracerebral Hemorrhage-Induced Acute Injury by Promoting Cell Ferroptosis. Front Neurol. 2021 Nov 12;12:763419. [Content Brief]
[2]. Ali I, et al. Protection by 20-5,14-HEDGE against surgically induced ischemia reperfusion lung injury in rats. Ann Thorac Surg. 2012 Jan;93(1):282-8. [Content Brief]
[3]. Jacobs ER, et al. Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices. Exp Cell Res. 2012 Oct 1;318(16):2143-52. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6209 mL | 13.1044 mL | 26.2089 mL | 65.5222 mL |
| 5 mM | 0.5242 mL | 2.6209 mL | 5.2418 mL | 13.1044 mL | |
| 10 mM | 0.2621 mL | 1.3104 mL | 2.6209 mL | 6.5522 mL | |
| 15 mM | 0.1747 mL | 0.8736 mL | 1.7473 mL | 4.3681 mL | |
| 20 mM | 0.1310 mL | 0.6552 mL | 1.3104 mL | 3.2761 mL | |
| 25 mM | 0.1048 mL | 0.5242 mL | 1.0484 mL | 2.6209 mL | |
| 30 mM | 0.0874 mL | 0.4368 mL | 0.8736 mL | 2.1841 mL | |
| 40 mM | 0.0655 mL | 0.3276 mL | 0.6552 mL | 1.6381 mL | |
| 50 mM | 0.0524 mL | 0.2621 mL | 0.5242 mL | 1.3104 mL | |
| 60 mM | 0.0437 mL | 0.2184 mL | 0.4368 mL | 1.0920 mL | |
| 80 mM | 0.0328 mL | 0.1638 mL | 0.3276 mL | 0.8190 mL | |
| 100 mM | 0.0262 mL | 0.1310 mL | 0.2621 mL | 0.6552 mL |
20-5,14-HEDGE Related Classifications
HY-148226 Related Classifications
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